【摘要】 目的探讨胃癌患者外周血、门静脉血及癌组织标本p53基因突变或异常表达的临床意义。方法应用PCR-SSCP法检测73例手术治疗的胃癌患者外周血和门静脉血中p53基因的突变,并用免疫组织化学法检测胃癌组织中p53蛋白的异常表达。结果73例胃癌患者中有7例检测到外周静脉血p53基因突变,有29例检测到门静脉血p53基因突变,有51例检测到胃癌组织中p53蛋白的异常表达;胃癌组织中的p53蛋白阳性表达率(69.86%)和门静脉血中p53基因突变检出率(39.73%)均显著高于外周血的检出率(9.59%)。经中位24个月随访,其中有16例出现术后异时性肝转移,这些患者外周静脉血、门静脉血以及胃癌组织中的p53基因突变检出率或p53蛋白阳性表达率均显著高于无肝转移者,而且7例外周静脉血、门静脉血及胃癌组织中同时检测出p53基因突变或p53蛋白阳性表达的患者均发生肝转移。结论胃癌患者外周血中p53基因突变者术后发生异时性肝转移的可能性显著增加,外周静脉血联合门静脉血以及胃癌组织中p53基因突变检测有助于判定胃癌患者的预后。更多还原

【Abstract】 Objective To investigate the clinical significance of detecting p53 gene mutation in blood of peripheral and portal vein or tumor tissue in patients with gastric carcinoma.Methods p53 gene mutation in peripheral and portal vein blood was detected by PCR-SSCP technology and p53 protein expression in tumor tissue was detected by immunohi- stochemical assay in 73 cases of gastric carcinoma.Results In 73 patients,p53 mutation rate were 9.6%(7/73)and 39.7% (29/73)in peripheral and portal vein blood(P<0.05).The positive rate of p53 gene mutation and abnormal expression of p53 protein in gastric cancer patients with liver metastasis were significantly higher than those without metastasis.Conclusion The detection of p53 gene mutation from peripheral or portal vein blood may be used to predict the metastasis and prognosis of gastric carcinoma.更多还原