【摘要】 目的:利用压力型整体体积描记箱和双室体积描记箱法,对常用的通过测定胸廓膨胀体积代替小鼠潮气量的测量方法进行评价。方法:选用14只中自主呼吸频率在90~120 b/min的6只小鼠,通过双室体积描记箱法进行潮气量和胸廓膨胀体积的同步测量。8只呼吸肌麻痹小鼠予以固定频率和潮气量通气,通过整体体积描记箱法测定胸廓膨胀体积。然后,对潮气量和胸廓膨胀体积进行比较和分析。结果:6只自主呼吸小鼠胸廓膨胀体积为(0.369±0.014)ml显著高于潮气量[(0.356±0.012)ml,P<0.01];8只呼吸肌麻痹小鼠胸廓膨胀体积为(0.233±0.003)ml显著低于呼吸机潮气量(0.3 ml,P<0.001)。结论:目前常用的以测定胸廓膨胀体积取代潮气量的方法不能准确测量潮气量,吸入气体的温化和湿化以及胸内负压使测得的胸廓膨胀体积大于潮气量。更多还原

【Abstract】 Objective: To evaluate the accordance of tidal volume(TV) with thoracic inflating volume(TIV) measured by pressure whole-body or double-chamber plethysmography in mice.Methods: TV and TIV by double-chamber plethysmography were simultaneously measured in 6 mice with spontaneous respiratory frequencies 90-120/min;TV and TIV by whole-body plethysmography were measured in 8 paralyzed mice ventilated by fixed frequency.Results: TIV value by double-chamber plethysmography was significantly higher than TV [(0.369±0.014)ml vs(0.356±0.012)ml,P<0.01)] in 6 spontaneously breathing mice.TIV value by whole-body plethysmography was significantly lower than TV[(0.233±0.003)ml vs 0.3 ml,P<0.001] in 8 paralyzed mice.Conclusion: The TV value assessed by TIV measurement can not be accurate,because of the humidifying and heating of inhaled air and negative thoracic pressure during the measurement.更多还原