【摘要】 目的:探讨1800 MHz手机辐射对人晶状体上皮细胞内活性氧(ROS)和DNA损伤的影响,以及叠加电磁噪声后对该效应的阻断作用。方法:体外培养的人晶状体上皮细胞受比吸收率(specific absorption rate,SAR)为4 W/kg的1800 MHz手机频段间断辐射24 h或与2μT电磁噪声联合作用24 h后,利用荧光探针检测ROS水平及用彗星试验检测DNA损伤水平。结果:4 W/kg手机辐射可明显诱发人晶状体上皮细胞内ROS及DNA损伤(P<0.001);与2μT电磁噪声联合暴露后可明显阻断该效应,与手机单独辐射相比,差异有显著性(P<0.001),但与假辐照组比较差异无显著性(P>0.05)。结论:电磁噪声可阻断1800 MHz手机辐射所诱发的晶状体上皮细胞内ROS及DNA损伤。更多还原

【Abstract】 Objective: To investigate whether the exposure to the electromagnetic noise can block reactive oxygen species(ROS) production and DNA damage of lens epithelial cells induced by 1800 MHz mobile phone radiation.Methods: The DCFH-DA method and comet assay were used respectively to detect the intracellular ROS and DNA damage of cultured human lens epithelial cells induced by 4 W/kg 1800 MHz mobile phone radiation or/and 2 μT electromagnetic noise for 24 h intermittently.Results: 1800 MHz mobile phone radiation at 4 W/kg for 24 h increased intracellular ROS and DNA damage significantly(P<0.05).However,the ROS level and DNA damage of mobile phone radiation plus noise group were not significant enhanced(P>0.05) as compared to sham exposure group.Conclusion: Electromagnetic noise can block intracellular ROS production and DNA damage of human lens epithelial cells induced by 1800 MHz mobile phone radiation.更多还原