【摘要】 目的:比较张家口产野生型和人工栽培型土三七药材中总酚酸的含量,并分析两者是否有显著性差异。方法:采用分光光度法,以没食子酸为对照品,以0.6%FeCl3-0.9%K3[Fe(CN)6](1∶0.9)混合溶液为显色剂,在720 nm波长处进行测定。结果:总酚酸分析的线性范围为0.228μg.mL-1~1.14μg.mL-1(r=0.9993),平均回收率为96.6%,RSD为1.9%(n=6)。野生型土三七中总酚酸含量为78.3 mg.g-1,RSD为5.5%(n=5)。人工栽培型土三七中总酚酸含量为82.4 mg.g-1,RSD为2.5%(n=5)。结论:野生型土三七和人工栽培型土三七中总酚酸含量无显著性差异。更多还原

【Abstract】 Objective:To analyze the content of total phenolic acid in wild and planted type Sedum Aizoon L.which were collected from Zhangjiakou.Methods:Total phenolic acid was analyzed by spectrophotometry at 720nm.The colour-developing agent was the mixture solution of 0.6% FeCl3-0.9% K3[Fe(CN)6](1∶0.9).Results: Calibration curve was constructed in the range of 0.228μg·mL-1~1.14 μg·mL-1(r=0.9993)for total phenolic acid.The average recovery was 96.6% for total phenolic acid and the RSD of the methods was 1.9%(n=6).The average contents were 78.3mg·g-1 for wild type(n=5)and 82.4mg·g-1 for planted type(n=5).The RSDs of the two methods were 5.5% and 2.5% respectively.Conclusion:Dealt with statistics,the determination of total phenolic acid had no significant difference between wild type and planted type Sedum Aizoon L.更多还原