【摘要】 目的:探讨丝裂霉素对胃癌细胞拓朴异构酶Ⅱα(TOPOⅡα)表达的影响。方法:人胃癌细胞株BGC-823培养,加入丝裂霉素后5h和10h后采集细胞,进行光、电镜观察和免疫组化染色。结果:5h组形态变化不明显,胞核中TOPOⅡα含量减少;10h组,细胞器减少,质膜破裂,其胞质与胞核中TOPOⅡα表达明显减少。结论:丝裂霉素对BGC-823癌细胞有明显的杀伤作用,其途径之一可能与抑制TOPOⅡα基因表达有关。更多还原

【Abstract】 Objective:To study the killing function of mitomycin(MMC) to gastric cancer cells and the expression of TopoⅡα.Methods:Human gastric cancer cellsBGC-823 was cultured,while MMC was added to culture continuously for 5 h or 10 h.Then the gastric cancer cells were observed under optical microscope and transmission electron microscope and stained with immunohistochemical method as well.Results:The morphology of gastric cancer cell was not changed significantly,but content of TopoⅡαin nuclear was reduced in the group for 5 hours.The changes in the group for 10 hours were as follows:the cell organ was obviously destroyed,the cell merabrane were burst,and the expression of TOPOⅡα was reduced significantly in cytoplasm and nuclear.Conclusion:Mitomyclin has killing fuction to gastric cancer cells,one of ways was to restrain the expression of TOPOⅡα gene.更多还原