【摘要】 目的探讨刚地弓形虫感染对小鼠睾丸细胞DNA的损伤作用。方法选用9～10周龄雄性BALB/c小鼠20只,随机分为对照组(CG)和3个染虫组(G1、G2和G3)。采用腹腔注射法建立小鼠刚地弓形虫感染的动物模型,CG组注射PBS 0.2 ml/只,染虫G1、G2和G3组各注射纯化刚地弓形虫速殖子悬液0.2 ml,剂量分别为2.5×10~3个、5×10~3个和1×10~4个。应用单细胞凝胶电泳(彗星实验)检测睾丸细胞DNA的损伤。结果对照组和3个染虫组的彗星实验尾矩分别为10.94±7.57、40.37±6.25、69.76±3.97和79.16±6.36;Olive尾矩分别为10.57±6.72、31.39±4.59、48.66±4.60和53.87±6.55,对照组和实验组彗星尾矩和Olive尾矩差异有统计学意义(P<0.01)。结论弓形虫感染可致小鼠睾丸细胞DNA不同程度的损伤。对小鼠有生殖遗传毒性。更多还原

【Abstract】 Objective To explore the adverse effect caused by infection of Toxoplasma gondii on the mice testicular cell.Methods Twenty BALB/c male mice(9—10 weeks old)were randomized into 4 groups,one negative control group(CG),three experimental group(G1:2.5×10~3,G2:5×10~3 and G3:1×10~4 tachyzoites).The negative control group was injected intraperitoneally with PBS and the experimental group was inoculated intraperitoneally with T.gondii tachyzoites(RH strain).All mice were sacrificed 6 days after infection and the testicular cells were applied onto single cell gel electrophoresis(comet assay)for detection of DNA damage.Results The tail moment mean value of the nega- tive control group was 10.94±7.57.Three experimental groups mean values of tail moment each were 40.37±6.25, 69.76±3.97 and 79.16±6.36.Significant difference in tail moment had been seen between the experimental groups and the negative control group(P<0.01).Similar difference in Olive tail moment were observed among the experimental groups and the negative control group(P<0.01).Conclusion T.gondii could cause DNA damage of mice testicular cell and have genetic genotoxicity to mice.更多还原