【摘要】 建立了CE法同时测定银黄胶囊中黄芩素、汉黄芩苷、汉黄芩素和绿原酸。采用未涂层弹性融硅石英毛细管柱,以40mmol/L硼砂溶液-15mmol/Lβ-环糊精(pH9.35)为运行缓冲液,分离电压12kV,重力进样,检测波长280nm。以对氨基苯甲酸为内标,黄芩素、汉黄芩苷、汉黄芩素、绿原酸的线性范围分别为2.5～12.5、10～80、1.25～10、15～120μg/ml;平均回收率分别为104.6%、97.2%、98.5%、95.8%,RSD分别为1.71%、2.91%、2.09%、2.83%。更多还原

【Abstract】 A CE method was established for the determination of baicalein, wogonoside, wogonin and chlorogenic acid in Yinhuang capsules. An uncoated fused silica capillary was used with 40mmol/L borax solution -15mmol/L β-CD buffer solution (pH 9.35) as running buffer at applied voltage of 12kV and detection wavelength of 280nm. The sample was injected by gravitational force. p-Aminobenzoic acid was used as the internal standard. The linear ranges of baicalein, wogonoside, wogonin and chlorogenic acid were 2.5 -12.5, 10 -80, 1.25 -10.0 and 15 -120μg/ml, respectively. The average recoveries were 104.6%, 97.2%, 98.5% and 95.8%, with RSDs of 1.71%,2.91%, 2.09%,2.83%, respectively.更多还原