【摘要】 目的:寻找简便、可重复的分子标记方法对绞股蓝属Gynostemma植物及其混淆品乌蔹莓Cayratia ja-ponica进行鉴别。方法:对7种常见药用绞股蓝属植物及其混淆品乌蔹莓的6个cpDNA片段进行PCR扩增,再利用TaqⅠ,HpaⅡ,EcoRⅠ,RsaⅠ,HhaⅠ,HindⅢ等6种限制性内切酶分别对扩增片段进行消化。结果:36种DNA片段/内切酶组合中,trnK1f-trnK2r片段与RsaⅠ组合可将乌蔹莓从绞股蓝属植物中区分出来,产物清晰、结果稳定。结论:PCR-RFLP分析方法可有效区分常见绞股蓝属植物与其混淆品乌蔹莓。更多还原

【Abstract】 Objective: To establish a convenient and effective method for the identification of Gynostemma and Cayratia japonica.Method: Eight species,including Gynostemm pentaphyllum,G.pentagynum,G.cardiospermum,G.longipe,G.yixingense,G.laxiflorum,G.guangxiense and C.japonica were investigated through PCR-RFLP of six chloroplast DNA fragments.The six gene fragments were digested by six restriction endonuclease respectively,including TaqⅠ,HpaⅡ,EcoRⅠ,RsaⅠ,HhaⅠ,HindⅢ.Result: Seven species of Gynostemma and their adulterant could be identified by trnK1f-trnK2r and Rsa.Conclusion: PCR-RFLP provides a quick,reliable molecular marker technique for identification of Gynostemma and their adulterant Cayratia japonica.更多还原