【摘要】 目的:采用高效液相色谱测定44种中药中1,2,3,4,6-五-O-倍酰-D-葡萄糖(1,2,3,4,6-penta-O-gal-loyl-D-glucose,PGG)的含量。方法:以乙醇-水溶液(50∶50)为提取剂,在50~60℃的温度下超声1 h,从中药中提取PGG;采用Diamonsil C18(4.6 mm×250 mm,5μm)色谱柱,流动相为乙腈-2.5%醋酸水溶液(18∶82),紫外检测波长280 nm,流速1.0 mL.min-1。结果:PGG在1~150μg.mL-1呈良好的线性关系(r=0.999 4)。五倍子、牡丹皮、赤芍、白芍等17种中药中均含有PGG,且在五倍子、牡丹皮、赤芍、白芍中PGG含量较高。结论:本实验用简单快捷的方法考察了44种中药中PGG的含量,并准确测定了五倍子等17种含PGG中药中PGG的含量,为从中药中分离、纯化PGG以及中药的开发利用提供了实验基础。更多还原

【Abstract】 Objective: To determine 1,2,3,4,6-penta-O-galloyl-D-glucose(PGG) in forty four kinds of Chinese traditional medicines by HPLC.Method: The PGG was extracted with a solvent consisting of ethanol and water(50∶50) in ultrasonic bath at 50-60 ℃ for 1 hour.A Diamonsil C18 column(4.6 mm×250 mm,5 μm) was used for the separation and analysis of PGG with a mobile phase consisting of acetonitrile and 2.5 % acetic acid solution(18∶82).The wavelength of detection was at 280 nm,and flow rate was set at 1.0 mL·min-1.Result: The calibration curve for PGG is linear over the range of 1-150 μg·mL-1(r=0.999 4),and PGG was found in seventeen kinds of Chinese traditional medicines,such as gall tree peony bark,red peony root,white peony root,and so on.Conclusion: The contents of PGG were determined in forty four kinds of Chinese traditional medicines by a rapid and precision HPLC method.更多还原