【摘要】 目的:利用HPLC-ELSD测定不同产地商陆药材中商陆皂苷A的含量以控制其质量。方法:采用HPLC-ELSD含量测定法,Phenom enex Luna C18(4.6 mm×250 mm,5μm)色谱柱,以甲醇-水-冰醋酸(68∶31∶1)为流动相。结果:商陆皂苷A在4.05~20.24μg呈线性,回收率为98.8%,RSD 1.4%。测定11个不同来源的药材,其商陆皂苷A的含量在0.32%~1.11%。结论:本法可快速、准确测定商陆药材中商陆皂苷A含量,可用于商陆药材的质量监控。更多还原

【Abstract】 Objective: To develop a HPLC-ELSD method for the quantitation of esculentosides A in Radix Phytolacca.Method: A Phenomenex Luna C18 column(4.6 mm×250 mm,5 μm) with a solvent system consisting of methanol-water-acetic acid(68∶31∶1) was used,and detected by ELSD.Result: The calibration curve of esculentosides A showed good linearity in the range of 4.05-20.24 μg,the average recovery was 98.8%,with RSD of 1.4%.11 batches of Radix Phytolaccae were analyzed,and the contents were 0.32% to 1.11 %.Conclusion: The method is simple,rapid and sensitive,can be used for determination of esculentosides A in Radix phytolaccae.更多还原