【摘要】 目的:研究蜜环菌菌种的复壮问题。方法:应用摇瓶扩繁无性后代的方法,观察几种培养基和不同培养方法对蜜环菌菌索形成的影响,并挑取新鲜粗壮菌索的先端部分进行斜面培养。结果:在富含有机氮的半固体培养基上蜜环菌最易形成粗壮的菌索。结论:选择适当的培养基及培养方法摇瓶扩繁蜜环菌后代,再进行选择培养是复壮蜜环菌菌种最简易的方法。更多还原

【Abstract】 Objective: To study the rejuvenation of Armillaria mellea strain.Method: Observe the influences of several ingredients and different cultural methods on the shoestring formation of the clonal offsprings in flask,and select the tip part of the shoestring for cultivation.Result: The strong shoestrings were easily restored in the semi-solid medium with rich organic nitrogen source.Conclusion: It was the easiest way to rejuvenate A.mellea strain by multiplying the clonal offsprings in a proper medium,together with the proper selection.更多还原