【摘要】 目的探讨糖皮质激素(GC)对大鼠骨密度(BMD)的影响。方法52只3.5月龄雌性SD大鼠随机分为基线组10只、糖皮质激素造模组(GCT)22只和对照组(Control)20只。基线组10只实验开始时即处死,GCT组和Control组分别在实验开始后1周、9周各处死11只和10只,应用QDR-4500A扇形束双能X射线吸收法(DXA)测量大鼠全身、离体股骨及其兴趣区(FROI)的BMD。结果①1周和9周GCT组的全身、离体股骨整体及兴趣区BMD均较Control组下降(1周时,FROI-1例外),其中,9周时,全身、股骨整体和FROI-5的BMD在两组间有显著性差异(P<0.05)。②大鼠造模9周后与Control组比较,骨量变化率从大到小依次为:FROI-2(-11.43%)、FROI-5(-9.83%)、FROI-7(-9.21%)、FROI-1(-8.71%)、股骨整体(-8.33%)、FROI-3(-8.12%)、FROI-4(-6.10%)、全身(-5.77%)、FROI-6(-5.18%)。③Control组随增龄出现全身、股骨整体及兴趣区BMD的增加,但GCT组BMD变化不大。结论GC所致骨丢失以松质骨含量丰富的兴趣区明显;GC可影响生长期大鼠骨量的获得。更多还原

【Abstract】 Objective To investigate the effect of glucocorticoid(GC) on bone mineral density(BMD) in rats.Methods Fifty-two 3.5-month-old female SD rats were recruited: ten of them were killed as the baseline group;and the others were randomly assigned to the GC-treated group(GCT) or control group,and each group included 22 rats or 20 rats.Whole body BMD in vivo,the BMD of femur and femoral regions of interest(FROI) were all measured by dual energy X-ray absorptiometry(DXA) using QDR-4500A equipment in the baseline group at the beginning,the first week and ninth week post-experiment in GCT and control groups respectively.Results ①Compared with the control rats at each time-point,there was a decrease in body BMD in vivo,the femoral BMD in vitro and FROI(at one week post-experiment,except FROI-1),but whole body BMD in vitro,the femoral BMD in vitro and FROI-5 in GCT at nine weeks post-experiment was significantly lower than that in the control group(P<0.05).②At the ninth week post-experiment,the change rate of bone mass was listed in order in GCT group as compared with the control group: FROI-2(-11.43%)>FROI-5(-9.83%)>FROI-7(-9.21%)>FROI-1(-8.71%)>femoral BMD in vitro(-8.33%)>FROI-3(-8.12%)>FROI-4(-6.10%)>whole BMD in vivo(-5.77%)>FROI-6(-5.18%).③Whole body BMD in vivo,the BMD of femur and femoral regions of interest(FROI) increased significantly in the control group with age,but there was no significant change in GCT group.Conclusions ①The regions of interest which are rich in trabecular bone are affected most markedly by glucocorticoid treatment.②Glucocorticoid may affect bone accumulation in the growing rats.更多还原