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EGF偶联白蛋白靶向纳米载体在荷瘤裸鼠体内分布的研究

In vivo Distribution of EGF-Conjugated Albumin Targeting Nanoparticles in Tumor-bearing Nude Mice

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【作者】 王树斌袁飞彭志平李少林孙世良罗弋宋琳

【Author】 WANG Shubin1, YUAN Fei2, PENG Zhiping1, LI Shaolin1, SUN Shiliang3, LUO Yi1, SONG Lin1 1Department of Radiology, Chongqing Medical University, Chongqing 400016, China 2Department of Biochemistry and Molecular Biology, Chongqing Medical University, Chongqing 400016, China 3The Municipal Cancer Hospital of Chongqing, Chongqing 400016, China

【机构】 重庆医科大学放射医学教研室重庆医科大学生物化学和分子生物学教研室重庆市肿瘤医院重庆医科大学放射医学教研室 重庆市400016重庆市400016

【摘要】 目的:本研究旨在探讨125I-EGF-BSA靶向纳米载体在荷人乳腺癌SK-BR3裸鼠体内的分布。方法:采用超声乳化-化学交联方法制备牛血清白蛋白纳米载体,经过羧和反应将与白蛋白纳米载体表面的活性氨基与EGF进行偶联反应,制备EGF偶联白蛋白纳米载体。在裸鼠体内进行荷人乳腺癌实验,将荷瘤裸鼠随机分为3个实验组,125I-BSA非纳米载体组(直接在BSA上标记125I),125I-EGF-BSA靶向纳米载体组和125I-BSA纳米载体组,检测不同时间点各组织的放射分布。结果:125I-EGF-BSA靶向纳米载体的瘤/血比值和瘤/肌肉比值明显高于其它两个给药组,差异有统计学意义(P<0.05)。125I-EGF-BSA靶向纳米载体在荷瘤裸鼠体内的放射性瘤/血比值和瘤/肌肉比值随时间逐渐升高,在2h达峰值,后略有下降。做方差分析,同一时间点125I-EGF-BSA靶向纳米载体的放射性瘤/血比值和瘤/肌肉比值均高于其他两个给药组,差异均具有统计学意义(P<0.05)。25I-EGF-BSA靶向纳米载体在肝、脾、肺等器官分布明显低于其它两组,差异有统计学意义(P<0.05)。结论:125I-EGF-BSA靶向纳米载体对裸鼠体内的人乳腺癌SK-BR3肿瘤具有明显的靶向性,而肝、脾、肺等器官的分布少。

【Abstract】 Objective: To explore the distribution of 125I-EGF-targeting nano-carrier BSA in nude mice bearing SK-BR3 human breast cancer. Methods: We used ultrasound emulsification-chemical cross-linking to prepare bovine serum albumin nanoparticles. Through a carboxymethyl reaction, the active amine group on the surface of albumin nanoparticles was coupled with EGF, forming EGF-conjugated albumin nanoparticles.Nude mice were used to establish the human breast cancer model and were randomly divided into three experimental groups: 125I-BSA non-nano-carrier group(125I directly linked to BSA), 125I-EGF-BSA targeting nano-carrier group, and 125I-BSA nano-carrier group. The radioactive distribution of the particles in different tissues was detected and calculated. Results: The tumor/blood ratio and tumor/muscle ratio in the 125I-EGF-BSA targeting nano-carrier group were higher than those in the other two groups(P<0.05). The distribution of 125I-EGF-BSA targeting nano-carrier in the liver, spleen, lungs and other organs was significantly lower than that in the other two groups(P<0.05). Conclusion: 125I-EGF-BSA targeting nano-carrier has obvious tumor-targeting effects on human breast cancer SK-BR3 in nude mice and is distributed to the liver, spleen, lungs and other organs less effectively.

【关键词】 EGF牛血清白蛋白纳米载体体内分布
【Key words】 EGFBovine serum albuminNano-carrierBody distribution
【基金】 国家自然科学基金资助(编号:30070230、30424005)
  • 【文献出处】 中国肿瘤临床 ,Chinese Journal of Clinical Oncology , 编辑部邮箱 ,2008年09期
  • 【分类号】R737.9
  • 【被引频次】9
  • 【下载频次】445
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