【摘要】 目的:检测新疆哈萨克族食管鳞癌患者中人乳头状瘤病毒(HPV)DNA的总感染率,通过统计学分析,探讨HPV感染与新疆哈萨克族食管鳞癌的相关性。方法:采用聚合酶链反应(PCR)技术检测了318例新疆哈萨克族食管鳞癌(ESCC)石蜡包埋组织中HPVDNA的感染情况,其中117例用直接裂解法制备样本DNA,201例用酚-氯仿-异戊醇提取DNA;PCR产物双向测序,测序结果应用BLAST在线分析,统计处理采用χ2检验,分析HPV与新疆哈萨克族食管鳞癌发生的相关性。结果:在新疆哈萨克族食管鳞癌组织中,HPVDNA总检出率为64.5%(205/318),其中直接裂解法组HPVDNA检出率为82.9%(97/117),酚-氯仿-异戊醇提取DNA组HPVDNA检出率为53.7%(108/201),二者之间差异有统计学意义(P<0.05)。按照病理分化程度对哈萨克族ESCC样本DNA中HPV总感染率进行分析发现,高分化ESCC中HPV阳性率为63.2%,中分化ESCC中HPV阳性率为69.8%,低分化ESCC中HPV阳性率为50.0%,三者之间差异无统计学意(P>0.05)。结论:HPVDNA感染与新疆哈萨克族食管鳞癌存在相关性,可能是新疆哈萨克族食管癌发生的重要因素之一。更多还原

【Abstract】 Objective: To evaluate the prevalence of HPV infection in esophageal squamous cell carcinoma(ESCC) in Kazakh of Xinjiang province. Methods: Polymerase chain reaction(PCR) was employed to screen HPV infection in samples from 318 ESCC cases. Using DNA extraction with phenol-Tris/chloroform and crude cell suspensions, we also compared the presence of HPV in the same samples. The correctness of PCR products of HPV DNA were demonstrated by random-sampled sequencing. The χ2 test was used to evaluate the difference in the distribution of HPV infection. Results: Of the 317 ESCC samples, 204(63%) were positive for the HPV L1 gene. The positive rate of HPV L1 gene was 53.5% in the group of DNA extraction with phenol-Tris/chloroform, and was 83%(97/117) in the group of crude cell suspensions, with a significant difference(P<0.05). No significant difference was found in HPV general infection rates among different pathological differentiations. Conclusion: HPV DNA infection is related to ESCC in Kazakh of Xinjiang province. It may play a role in the occurrence and development of ESCC.更多还原