【摘要】 目的采用HPLC-DAD中梯度洗脱和检测波长时间序列采样的方法,建立甘草(Glycyrrhiza uralensis Fisch.)中4种黄酮成分(甘草苷、异甘草苷、甘草素、异甘草素)和3种甘草皂苷成分(甘草酸、甘草皂苷G2、乌拉尔甘草皂苷B)的同时分析方法。方法采用C18(4.6 mm×250 mm,5μm)色谱柱。流动相:乙腈-(0.5%醋酸铵+1%冰醋酸),梯度洗脱0~12 min从6∶94到22∶78,12~25 min从22∶78到25∶75,25~45 min从25∶75到40∶60,45~50 min从40∶60到6∶94,保持5 min;检测波长:时间序列采样。结果7种成分的线性关系良好,平均加样回收率在98%~103%之间。结论该方法准确、灵敏度高,可用于甘草药材质量的评价。更多还原

【Abstract】 OBJECTIVE To establish the method for simultaneous analysis of four flavone components(liquiritin,isoliquiritin,liquiritigenin,isoliquiritigenin) and three saponin components(glycyrrhizin,licorice-saponin G2 and uralsaponin B) in Glycyrrhiza uralensis Fisch.using gradient elution and timetable detected wavelength by HPLC-DAD.METHODS The chromatographic separation was performed on a C18(4.6 mm×250 mm,5 μm) column eluted with a mobile phase of acetonitril-(0.5%NH4Ac+1%HAc),gradient program used was as follows:0-12 min 6∶94 linear change to 22∶78,12-25 min 22∶78 linear change to 25∶75,25-45 min 25∶75 linear change to 40∶60,45-50 min 40∶60 linear change to 6∶94,and this was maintained for 5 min and time-table detected wavelength.RESULTS Seven components had nice linear relation between the peak area and injected concentration.The average recoveries of the seven components were in the range from 98% to 103%.CONCLUSION The method was accurate,and sensitive.It′s suitable to determine seven active components in G.uralensis and can be used for evaluating the quality of G.uralensis.更多还原