【摘要】 目的建立大鼠血浆中芍药内酯苷的HPLC-MS/MS定量分析方法,并用于大鼠体内芍药内酯苷药动学研究。方法血浆样品采用固相萃取预处理。色谱柱为Symmetry C18(4.6mm×50mm,3.5μm),流动相为乙腈-0.05%甲酸溶液(25∶75)。采用三重四极杆质谱仪进行多反应监测方式(MRM),电喷雾离子源。对所建方法进行了较全面的验证。考察了3种剂量芍药内酯苷给与大鼠后的药动学特征。结果所建方法灵敏、重现性良好。芍药内酯苷在体内吸收迅速,消除也较快。经统计学检验表明,在给定的3个剂量范围内,芍药内酯苷具有线性动力学特征。结论建立的方法可用于芍药内酯苷的药动学研究。更多还原

【Abstract】 OBJECTIVE To develop a rapid,sensitive and selective liquid chromatography tandem spectrometry method for the determination of albiflorin in rat plasma,and to investigate its pharmacokinetic parameters in rats.METHODS The plasma samples were pretreated with solid phase extraction.HPLC analysis was carried out on a Symmetry C18 column(4.6 mm×50 mm,3.5 μm)with a mobile phase consisting of acetonitrile and 0.05% formic acid(25∶75).Detection was carried out on a triple quadrupole tandem mass spectrometer by multiple-reaction monitoring and an electrospray ionization source was employed as the ionization source.The method was fully validated.The phamacokinetic characteristics of albiflorin was investigated after oral administration to rats in three dosages.RESULTS The method was sensitive and reproducable.Albiflorin was Absorbed and eliminated rapidly.The statistic analysis showed the pharmacokinetics of albiflorin complied with linear kinetic course.CONCLUSION The method could be applied to the study of pharmacokinetics of albiflorin.更多还原