【摘要】 目的探讨EseroS-GS对脂多糖(LPS)诱导小鼠腹腔巨噬细胞核因子-kB(NF-kB)活化及炎性细胞因子肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)、白细胞介素-6(IL-6)基因表达的调节,为Eseros-GS的临床运用提供理论依据。方法分别用LPS或Eseros-GS+LPS处理体外培养的小鼠巨噬细胞,采用蛋白质印迹分析和电泳迁移率改变分析法(EMSA)检测细胞中NF-kB活性,用逆转录-聚合酶链反应(RT-PCR)和酶联免疫吸附法(ELISA)检测细胞中TNF-α,IL-1β、IL-6 mRNA和蛋白的表达。结果LPS组NF-kB活性和TNF-α、IL-1β、IL-6含量在刺激后2～12h明显高于正常对照组(P<0.01),而EseroS-GS+LPS组NF-kB活性和TNF-α、IL-1β、IL-6含量均显著低于LPS组(P<0.01)。结论结果提示,LPS可诱导巨噬细胞NF-kB活化,导致TNF-α、IL-1β、IL-6基因表达增强,而EseroS-GS能抑制NF-kB活化而调节TNF-α、IL-1β、IL-6基因的表达。更多还原

【Abstract】 OBJECTIVE To investigate the activation of nuclear factor-kB(NF-KB)and the expression of inflammatory cytokines in lipopolysaccharide-induced murine peritoneal macrophages stimulated with 2-S[2-(N-carbonyl-3-β-aminoethyl-5-hydroxyindole)-1- (α-tocopheryl-6-yloxy-carbonyl)ethyl]glutathione(EseroS-GS).METHODS Peritoneal macrophages cultured in vitro were given with LPS or EseroS-GS+LPS.The NF-kB activity of peritoneal macrophages was tested by Western blotting and electrophoretic mobility shift assay(EMSA).The expression of TNF-α,IL-1β,IL-6 in peritoneal macrophages were measured by reverse transcription polymerase chain reaction techniques(RT-PCR)and enzyme-linked immuno sorbent assay(ELISA).RESULTS The activity of NF- kB and the level of TNF-α,IL-1β,IL-6 was significantly increased after LPS stimulation for 2 to 12 h(P<0.01).Compared with LPS- stimulated group,both NF-kB activity and concentration of TNF-α,IL-1β,IL-6 were significantly lowered in EseroS-GS+LPS group (P<0.01).CONCLUSION LPS Might activate NF-kB in peritoneal macrophages and induce the increase of transcription and expression of TNF-α,IL-1β,IL-6 genes.EseroS-GS could inhibit the activation of NF-kB and regulate the expression of TNF-α,IL-1βand IL-6 genes in peritoneal macrophages.更多还原