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EseroS-GS对脂多糖诱导小鼠腹腔巨噬细胞炎性因子生成的调节

Effects of EseroS-GS on Production of Pro-Inflammatory Factors from Lipopolysaccharide Activated Murine Peritoneal Macrophages

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【作者】 张申尹利华卫涛涛

【Author】 ZHANG Shen~1 YIN Li-hua~1 WEI Tao-tao~2 (1.Department of Laboratory Medicine,Huaihua Medical College,Huaihua 418000,China; 2.Center for Structural and Molecular Biology,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China)

【机构】 怀化医学高等专科学校医学检验系中国科学院生物物理研究所结构与分子生物学研究中心

【摘要】 目的探讨EseroS-GS对脂多糖(LPS)诱导小鼠腹腔巨噬细胞核因子-kB(NF-kB)活化及炎性细胞因子肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)、白细胞介素-6(IL-6)基因表达的调节,为Eseros-GS的临床运用提供理论依据。方法分别用LPS或Eseros-GS+LPS处理体外培养的小鼠巨噬细胞,采用蛋白质印迹分析和电泳迁移率改变分析法(EMSA)检测细胞中NF-kB活性,用逆转录-聚合酶链反应(RT-PCR)和酶联免疫吸附法(ELISA)检测细胞中TNF-α,IL-1β、IL-6 mRNA和蛋白的表达。结果LPS组NF-kB活性和TNF-α、IL-1β、IL-6含量在刺激后2~12h明显高于正常对照组(P<0.01),而EseroS-GS+LPS组NF-kB活性和TNF-α、IL-1β、IL-6含量均显著低于LPS组(P<0.01)。结论结果提示,LPS可诱导巨噬细胞NF-kB活化,导致TNF-α、IL-1β、IL-6基因表达增强,而EseroS-GS能抑制NF-kB活化而调节TNF-α、IL-1β、IL-6基因的表达。

【Abstract】 OBJECTIVE To investigate the activation of nuclear factor-kB(NF-KB)and the expression of inflammatory cytokines in lipopolysaccharide-induced murine peritoneal macrophages stimulated with 2-S[2-(N-carbonyl-3-β-aminoethyl-5-hydroxyindole)-1- (α-tocopheryl-6-yloxy-carbonyl)ethyl]glutathione(EseroS-GS).METHODS Peritoneal macrophages cultured in vitro were given with LPS or EseroS-GS+LPS.The NF-kB activity of peritoneal macrophages was tested by Western blotting and electrophoretic mobility shift assay(EMSA).The expression of TNF-α,IL-1β,IL-6 in peritoneal macrophages were measured by reverse transcription polymerase chain reaction techniques(RT-PCR)and enzyme-linked immuno sorbent assay(ELISA).RESULTS The activity of NF- kB and the level of TNF-α,IL-1β,IL-6 was significantly increased after LPS stimulation for 2 to 12 h(P<0.01).Compared with LPS- stimulated group,both NF-kB activity and concentration of TNF-α,IL-1β,IL-6 were significantly lowered in EseroS-GS+LPS group (P<0.01).CONCLUSION LPS Might activate NF-kB in peritoneal macrophages and induce the increase of transcription and expression of TNF-α,IL-1β,IL-6 genes.EseroS-GS could inhibit the activation of NF-kB and regulate the expression of TNF-α,IL-1βand IL-6 genes in peritoneal macrophages.

【基金】 怀化市科技计划资助项目(0502)
  • 【文献出处】 中国药学杂志 ,Chinese Pharmaceutical Journal , 编辑部邮箱 ,2008年14期
  • 【分类号】R96
  • 【被引频次】1
  • 【下载频次】108
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