节点文献
重组病毒巨噬细胞炎性蛋白在大鼠体内的药动学及组织分布
Pharmacokinetics and Tissue Distribution of Recombinant vMIP in Rats
【摘要】 目的了解重组病毒巨噬细胞炎性蛋白(vMIP)在SD大鼠体内的药动学及组织分布。方法对vMIP进行碘标、分离纯化和鉴定,然后利用其进行药动学实验。采用同位素示踪法测量vMIP在SD大鼠体内的药动学,并检测其在大鼠组织中的分布。结果60,240μg·kg-12个剂量组(静脉注射给药)的主要药动学参数分别为ρmax(249·4±84·50),(887·01±204·22)μg·L-1;t1/2β(1·5±0·28),(2·65±0·85)h;AUC0-∞(136·0±40·70),(493·84±49·42)μg·h·L-1。tmax10·0min。结论vMIP进入大鼠体内主要分布在肝和肺,在体内消除较快,其动力学特征符合一级吸收二室开放模型。
【Abstract】 OBJECTIVE To investigate the pharmacokinetics and tissue distribution of recombinant vMIP in SD rats.METHODS The vMIP was prepared by radioiodine label,then separated,purified and identified with the 125I-vMIP.The 125I-vMIP was determined and tissue distribution of vMIP was discribed in SD rats.RESULTS The main pharmacokinetic parameters of the low,middle(60 μg·kg-1) and high dosage(240 μg·kg-1) groups(after intra-venous administration) were as follow:ρmax(249.4±84.50),(887.01±204.22) μg·L-1;t1/2β(1.5±0.28),(2.65±0.85) h ;AUC0-∞(136.0±40.70),(493.84±49.42) μg·h·L-1.t max10.0 min.CONCLUSION vMIP was distributed mostly in lung and liver.The vMIP was eliminated rapidly in rats.The pharmacokinetic profile was described by a 2-compartment open model with the first order absorption.
【Key words】 viral macrophage inflammatory protein; pharmacokinetics; tissue distribution;
- 【文献出处】 中国药学杂志 ,Chinese Pharmaceutical Journal , 编辑部邮箱 ,2008年11期
- 【分类号】R96
- 【被引频次】1
- 【下载频次】141