【摘要】 目的制备槲皮素固体脂质纳米粒(QT-SLN),并考察其理化性质及小鼠口服吸收特性。方法以具有生物相容性的硬脂酸作为脂质载体,采用高温乳化-低温固化法制备槲皮素固体脂质纳米粒,以均匀设计法优化处方与制备工艺;透射电镜(TEM)观察其微观形态,并测定其粒径分布;建立测定纳米粒和胃肠道内容物及粪样混合物中槲皮素的紫外分光光度法。结果槲皮素固体脂质纳米粒为球状或类球状,平均粒径为217.3 nm,包封率为48.50%,其小鼠体内吸收优于槲皮素原料药。结论高温乳化-低温固化法适于制备槲皮素固体脂质纳米粒,该制剂促进了槲皮素的小鼠口服吸收。更多还原

【Abstract】 OBJECTIVE To prepare quercetin-loaded solid lipid nanopanicles(QT-SLN)and investigate its properties and the o- ral absorption character in mice.METHODS QT-SLN was prepared by the method of emulsion evaporation at a high temperature and solidification at a low temperature.Stearic acid with high biocompatibility was used as lipid carrier.The optimum formulation was se- lected by uniform design.Its morphology was examined by transmission electron microscope(TEM)and the particle diameter distribu- tion was decided.A UV-vis analytical method for the determination of quercetin in the nanoparticles and in the mixture of gastrointesti- nal contents and feces was established.RESULTS QT-SLN was spherical shape with mean diameter of 217.3 nm and entrapment ef- ficiency(EE)of 48.50%.The oral absorption of QT-SLN in mice was better than that of quercetin suspension.CONCLUSION QT- SLN can be prepared by the method of emulsion evaporation at a high temperature and solidification at a low temperature,and it also can improve the oral absorption of quercetin in mice.更多还原