【摘要】 采用高效液相色谱建立了伪品血竭中苏丹红Ⅳ的鉴别和含量测定方法。含量测定采用Agilent Eclipse Plus C18色谱柱,柱温30℃,流动相为乙腈-水(95∶5),流速为1.0mL·min-1,检测波长为520nm;液-质联用验证采用Merck Chromolith Performance RP-18e色谱柱,流动相为乙腈-0.1%的甲酸溶液(95∶5),ESI+扫描模式。供试样品中相关分子离子峰及碎片特征与苏丹红Ⅳ对照品一致,含量测定苏丹红Ⅳ在0.0849~3.3975μg的范围内线性关系良好(r=0.9999),回收率为99.82%。该方法快速、简便,易行,具有良好的专属性、稳定性和重复性。更多还原

【Abstract】 To develop a specific method for identification and determination of SudanⅣ in counterfeit drug of Sanguis Draconiis. HPLC-DAD and LC-MS methods were established for the separation and detection. The HPLC system consisted of Agilent Eclipse Plus C18 column,by using acetonitrile and water (95∶5) in HPLC-DAD method, and acetonitrile and 0.1% fomic acid (95∶5) in LC-MS/MS method as the mobile phase separately. SudanⅣ had good linearity in the range of 0.0849～3.3975 μg (r=0.9999). The average recovery was 99.82%. This method is rapid, simple and accurate for quantitative analysis of Sanguis draconiis, and has good specificity, stability and repeatability.更多还原