【摘要】 目的观察异丙酚对大鼠在体缺血/再灌注损伤心肌m-钙激活蛋白酶(m-calpain)激活和线粒体膜通透性转运的影响,探讨异丙酚对心肌缺血/再灌注损伤的保护机制。方法选用健康SD雄性大鼠24只,随机分为3组(n=8),假手术组(Sham组)、缺血/再灌注组(I/R组)、异丙酚组(P组)。分别在结扎左冠状动脉(LV)前即刻(T1)、缺血30min即刻(T2)及LV松开再灌注120min即刻(T3)3个时间点抽取动脉血2ml检测血浆超氧化物岐化酶(SOD)活性、丙二醛(MDA)和肌钙蛋白I(cTnI)浓度,免疫组织化学SP法检测各组大鼠m-calpain水平。结果与T1时间点相比,T2、T3时间点的血浆SOD活性降低(P<0.05),MDA及cTnI浓度升高(P<0.05);与T2时间点相比,T3时间点的血浆SOD活性降低(P<0.05),MDA及cTnI浓度升高(P<0.05)。在T2时间点,与I/R组相比,P组的血浆SOD活性升高(P<0.05),MDA及cTnI浓度降低(P<0.05);在T3时间点,与I/R组相比,P组的血浆SOD活性升高(P<0.05),MDA及cTnI浓度降低(P<0.05)。与对照组相比,I/R组和P组m-calpain水平均升高,与I/R组相比,P组m-calpain水平降低(P<0.05)。结论异丙酚对缺血/再灌注损伤心肌具有保护作用,通过抑制心肌缺血/再灌注损伤过程中m-calpain的激活而减轻缺血/再灌注损伤时线粒体膜通透性转运是其保护机制之一。更多还原

【Abstract】 Objective To observe the effects of propofol on the activation of m-calpain and the mitochondrial permeability transition of ischemia-reperfusion myocardial myocytes. Methods 24 healthy male SD rats weighing 250~350 mg were divided into 3 groups (n =8 each):(1)control group,(2)ischemia/reperfusion(I/R) group,(3)propofol group. Blood sample was taken from femoral artery at three time points to assay the concentration of MDA,SOD and cTnI. Results Compared with T1,the SOD activity at T2~T3 was lower(P < 0.05),and the MDA concentration and cTnI level were higher(P < 0.05)in both groups. Compared with T2,the SOD activity was lower(P < 0.05)at T3,and the concentration of MDA and cTnI level were higher(P < 0.05)in both groups. Compared with I/R Group,at the T2 and T3 points,the SOD activity was higher in P Group(P < 0.05),and MDA concentration and cTnI level were lower in P Group(P < 0.05); the SOD activity in P Group at T3 was higher than that of I/R group(P < 0.05),and the concentration of MDA and cTnI level were lower(P < 0.05). Conclusion To inhibit the activation of m-calpain in ischemia-reperfusion injury cadiocytes and decrease the MPT is one of the protective mechanisms of propofol on myocardium which have undergone ischemia-reperfusion injury.更多还原