【摘要】 目的探讨肿瘤坏死因子α(TNF-α)在酒精性肝病大鼠肝脏中的表达及抗纤复方Ⅰ号(KXⅠ)对其表达的影响。方法Wistar大鼠给予乙醇8～10g·kg-1·d-1,3次灌胃,共12周,制备酒精性肝病大鼠模型为酒精组,对照组每只大鼠给予生理盐水2.0ml,KXⅠ组在给予同酒精组等量乙醇的基础上,给予KXⅠ水煎剂4.0ml.kg-1·d-1,2次灌胃,共12周。实验4,8,12周末分批处死对照组、酒精组、KXⅠ组大鼠,HE染色观察肝脏组织学改变。免疫组织化学技术检测TNF-α在酒精性肝病过程中的定位及表达,并用病理图像分析仪对其结果半定量分析。结果酒精组12周末,肝小叶脂肪变,肝细胞坏死增多,炎性细胞浸润明显;KXⅠ组肝细胞坏死及炎性细胞浸润不明显。对照组的正常肝脏组织,肝细胞的胞浆中散在有TNF-α弱表达,酒精组4周末开始,以中央静脉为中心,肝细胞胞浆中TNF-α表达增多,且随着乙醇刺激时间的延长,肝细胞胞浆中TNF-α的表达呈进行性增加,差异显著(P<0.01)。结论酒精性肝病过程中,TNF-α表达进行性增强,KXⅠ能抑制TNF-α的表达,延缓肝纤维化的形成和发展。更多还原

【Abstract】 Objective To investigate the expression of tumor necrosis factor-α(TNF-α)in rats with alcoholic liver disease and the effect of Kang xian fu fang Ⅰ(KXⅠ)on TNF-α expression.Methods Wistar rats weighing 160 g to 190 g were lavaged with normal saline(2.0 ml),ethanol(8~10 g·kg-1·d-1),and ethanol plus KXⅠ(4.0 ml·kg-1·d-1)for 12 weeks in control group,alcohol group and KXⅠ group,respectively.At the end of 4,8,12 weeks,the rats were killed,and the histopathological changes of the liver were observed.The TNF-α expression in the liver was determined by immunohistochemical method.Results At the end of 12 weeks,steatosis,inflammation and necrosis were observed in alcohol group.In contrast,these alterations were attenuated by KXⅠ administration.In normal liver,TNF-α expression was seen in the cell plasma of liver,whereas the expression of TNF-α was seen around the central vein from the end of week 4.Conclusion In rats with alcoholic liver disease,the expression of TNF-α is increasingly increased,and KXⅠ can decrease TNF-α expression.更多还原