【摘要】 目的了解当地莱姆病的主要生物媒介—蜱的种类、带菌状况以及在传播莱姆病中的作用。方法采用布旗法及动物诱捕法在山林地区采集蜱,对蜱种进行鉴定。并进行病原分离培养和PCR检测。结果共收集到1226只蜱,经鉴定属于硬蜱科中2属2种:硬蜱属的全沟硬蜱和血蜱属的长角血蜱。其中以长角血蜱为优势种,构成比占92.58%(1135/1226);随机对300只长角血蜱进行针对莱姆病螺旋体的PCR检测,有14只阳性,阳性率为4.67%。并对其余926只蜱进行病原分离培养,未得到莱姆病螺旋体。结论血蜱可能是天津蓟县地区莱姆病传播的主要生物媒介。更多还原

【Abstract】 Objective To explore the species,bacteria carrying status and the role in causing Lyme disease of ticks.Methods ① Flagging and trapping methods were used to collect ticks in forest area and then the species of captured ticks were identified.② BSK Ⅱ culture medium was used to isolate the pathogen.③ PCR was used to detect the germ-carrying rate of ticks.Results A total of 1 226 ticks were collected,and then were indentified as Ixodidae(2 genera,2 species).Haemaphysalis longicornis was the dominant specie.The carrying-rate of Haemaphysalis longicornis in ticks was 4.67% by PCR,but no pathogen was found by isolation or culture.Conclusion Haemaphysalis longicornis was an important vector in Ji County of Tianjin city.更多还原