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硒化壳聚糖对NB4细胞的作用及其与NF-κB信号分子的关系

Effect of Selenium Chitosan on the NB4 Cells and Its Correlation with NF-κB Signaling Molecules

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【作者】 邓守恒卢方安邓守明杨均

【Author】 DENG Shou-heng,LU Fang-an(Basic Medical College,Yunyang Medical College,Shiyan 442000,China)DENG Shou-heng,YANG Jun(The Affiliated People’s Hospital of Yunyang Medical College,Shiyan 442000,China)DENG Shou-ming(Danjiangkou People’s Hospital,Teaching Hospital of Yunyang Medical College,Dan-jiangkou 442700,China)

【机构】 郧阳医学院基础医学院郧阳医学院教学医院暨丹江口市人民医院郧阳医学院附属人民医院

【摘要】 目的:研究硒化壳聚糖对人急性早幼粒细胞性白血病NB4细胞抗氧化指标的影响及其与核因子κB(NF-κB)信号分子的关系。方法:取NB4细胞加入不同浓度的硒化壳聚糖(50、100、200mg·L-1)作用24h后取上清检测谷胱甘肽过氧化物酶(GSH-px)、超氧化物歧化酶(SOD)、丙二醛(MDA)、NF-κB蛋白含量或活性,并设培养液为对照组。结果:与对照组比较,硒化壳聚糖各浓度组GSH-px活性明显升高(P<0.01),SOD活性和MDA含量明显降低(P<0.01或P<0.05),NF-κB蛋白含量明显降低(P<0.01),并呈量效关系。结论:硒化壳聚糖可通过减少进入细胞核内NF-κB蛋白含量的方式下调其下游基因表达,以及增强细胞抗氧化能力的方式抑制NB4细胞增殖,诱导细胞凋亡。

【Abstract】 OBJECTIVE: To study the effect of selenium chitosan on anti-oxidative ability in NB4 cells in patients with acute promyelocytic leukemia(APL) and its correlation with the NF-κB signaling molecules.METHODS: NB4 cells were treated with different concentrations of selenium chitosan(50,100,200 mg·L-1) for 24 h before the supernatant was taken for the determination of levels of GSH-px,SOD,MDA,and NF-κB signal protein molecules,with culture solution set as control.RESULTS: Compared with the control group,exposure of NB4 cells to different concentrations of selenium chitosan for 24 h could significantly increase GSH-px activity(P<0.01),decrease SOD activity and MDA content(P<0.01 or P<0.05),and decrease the level of NF-κB in obvivous dose-effect relationship(P<0.01).CONCLUSION: Selenium chitosan downregulates gene expresxion of its downstream by reducing the level of NF-κB entering into nucleus and inhibits the proliferation of NB4 cells and induces apoptosis by enhancing the anti-oxidative ability of cells.

【关键词】 硒化壳聚糖抗氧化NB4细胞NF-κB
【Key words】 Selenium chitosanAntioxidationNB4 cellsNF-κB
【基金】 湖北省教育厅基金资助项目(B200624004);湖北省十堰市科学技术局基金资助项目(十科通[2006]18号)
  • 【分类号】R733.7
  • 【被引频次】2
  • 【下载频次】88
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