【摘要】 实验主要探讨了GW9662对过氧化物酶体增殖物激活受体(γPPAR)γ的拮抗作用,以及PPARγ被拮抗后共轭亚油酸(CLA)对淋巴细胞炎性细胞因子TNF-α和IL-6分泌的影响。实验1设置5个处理:淋巴细胞培养体系中GW9662添加量分别为0、5、10、20μmol/L和40μmol/L。实验2设6个处理:空白对照组、20μmol/L的GW9662组、CLA处理组(c9,c11-CLA或t10,c12-CLA各100μmol/L)、CLA(c9,t11-CLA或t10,c12-CLA各100μmol/L)+20μmol/L GW9662处理组。结果表明:淋巴细胞PPARγ活性呈剂量依赖性地被GW9662抑制(P<0.05),而TNF-α和IL-6的分泌则随GW9662添加剂量的增加呈线性或二次曲线变化趋势(P<0.05)。这种变化随着培养体系中CLA的添加而得到缓解,即与20μmol/L GW9662处理组相比,CLA+GW9662处理组TNF-α和IL-6的分泌量显著降低(P<0.05),而与CLA处理组相比,TNF-α和IL-6的分泌量则无显著变化。从本实验可得出,PPARγ活性被GW9662抑制后,添加CLA同样可抑制淋巴细胞炎性细胞因子TNF-α和IL-6的分泌。更多还原

【Abstract】 This experiment investigated the effects of conjugated linoleic acid(CLA)and PPARγ antagonists GW9662 on the activity of peroxisome proliferator-activated receptor γ(PPARγ) and the production of TNF-and IL-6 in lymphocytes.In the Exp.1,the lymphocytes were divided into 5 treatments: additions dose of GW9662 were 0,5,10,20 μmol/L and 40 μmol/L,respectively.In the Exp.2,the lymphocytes were divided into 6 treatments: control group,20 μmol/L GW9662 group,CLA-treated group(100 μmol/L c9,t11-CLA or t10,c12-CLA,respectively),20 μmol/L GW9662 + 100 μmol/L CLA(c9,t11-CLA or t10,c12-CLA) group.The results showed that the activity of PPARγ was decreased with the addition of GW9662 in a concentration-dependent manner,while the production of TNF-α and IL-6 were linearly and quadratically increased(P < 0.05).However,addition of CLA reversed the effect of GW9662.Thus we can draw a conclusion that CLA can still inhibit the secretion of TNF-α and IL-6 after the activity of PPARγ was suppressed by GW9662.更多还原