【摘要】 目的:观察中国苏皖地区汉族老年(≥60岁)人群KLOTHO基因第4外显子C-1818T多态性分布,探讨其与老年人急性冠脉综合征(ACS)的可能关系。方法:采用聚合酶链反应及基因芯片技术,检测272例老年ACS患者(ACS组)和139例老年非冠心病者(对照组)的KLOTHO基因C-1818T多态性。结果:(1)与对照组相比:ACS组CT基因型频率明显升高,差异有统计学意义(30.9vs20.9,P=0.031),CC、TT基因型及T等位基因频率差异均无统计学意义(分别为72.7vs65.4、6.5vs3.7和16.9vs19.1,P值均>0.05)。(2)将ACS组按性别进行进一步分析后发现,分别与对照组相比:①男性CT基因型频率明显升高,差异有统计学意义(28.5vs16.7,P=0.041),CC、TT基因型及T等位基因频率差异无统计学意义(分别为75.6vs68.5、7.7vs3.0和16.0vs17.3,P值均>0.05);②女性CC、CT、TT基因型及T等位基因频率差异无统计学意义(分别为68.9vs56.9、26.2vs37.5、4.9vs5.6和18.0vs24.3,P值均>0.05)。在调整相关危险因素并经多元Logistic回归分析后,与对照组相比:ACS组(优势比=1.993,95可信区间=1.180-3.367,P=0.010)和ACS组男性患者(优势比=2.156,95可信区间=1.010-4.602,P=0.047)CT基因型频率均显著升高,差异有统计学意义。结论:在中国苏皖地区汉族老年人群(特别是老年男性)中,ACS患者CT基因型频率升高,提示KLOTHO基因C-1818T多态性CT基因型与老年人特别是老年男性ACS的发病存在相关性。更多还原

【Abstract】 Objective:To study the distribution of KLOTHO gene C-1818T polymorphism in the Chinese Han population of Suwan region and to explore the possible associations of the KLOTHO gene C-1818T polymorphism with acute coronary syndrome(ACS) in the elderly(age≥60 years).Methods:This study was conducted with a case-control design including 272 patients with ACS(ACS group) and 139 control subjects who were free from coronary artery disease through coherence checks(control group).The KLOTHO gene C-1818T polymorphism was determined by polymerase chain reaction and gene microarray.Results:(1)As compaired with the control group,the prevalence of the CT genetype in elderly patients of ACS group was obveriously higher(20.9% vs 30.9%,P=0.031).But the frequencies of CC、TT genotype and the T allele of the C-1818T in ACS group were not significantly different(72.7% vs 65.4%、6.5% vs 3.7%、16.9% vs 19.1%,respectively,P>0.05).(2)After subgrouped by sex we found that as compaired with the control group:①The CT genetype in male group of ACS was also obveriously higher(16.7% vs 28.5%,P=0.041),the frequencies of CC、TT genotype and the T allele in male group were not significantly different(75.6% vs 68.5%、7.7% vs 3.0%、16.0% vs 17.3%,respectively,P>0.05).②Also the frequencies of CC、CT、TT genotype and the T allele in male group were not significantly different(68.9% vs 56.9%、26.2% vs 37.5%、4.9% vs 5.6%、18.0% vs 24.3%,respectively,P>0.05).After adjustment for conventional risk factors by multiple logistic regression analysis,the carrier prevalence of the CT genetype was still differ significantly between elderly(odds ratio=1.993,95% confidence interval was 1.180-3.367,P=0.010),especially in elderly male patients(odds ratio=2.156,95% confidence interval was 1.010-4.602,P=0.047) with ACS and control subjects.Conclusion:The CT genetype of KLOTHO gene C-1818T polymorphism was associated with the susceptibility to ACS in the elderly,especially the male,in the Chinese Han population of Suwan region.更多还原