【摘要】 利用农杆菌介导转化法,将棉纤维特异表达启动子GAE6-3A驱动的兔角蛋白基因(KAP)转入常规棉花"冀合713"中。PCR及Southern杂交分析表明,兔角蛋白基因已整合进棉花基因组。6个转基因株系中,4个为单拷贝插入,转基因后代的分离符合孟德尔3∶1的1对基因的分离规律,外源基因在这些转基因后代中能稳定遗传。对这4个转基因株系T3代纤维检测结果表明,兔角蛋白基因对棉花纤维品质的影响主要是纤维强度的提高,其中3个株系纤维强度提高率达10%以上。更多还原

【Abstract】 Through Agrobacterium-mediated transformation, a rabbit keratin gene driven by a fiber-specific promoter GAE6-3A, was introduced into upland cotton cultivar "Jihe 713". PCR analysis and Southern bolt indicate integration of exogenous rabbit keratin gene into "Jihe 713" genome. The inserted exogenous gene are mono-copies in four of the six cases, and the separation of transgenic progenies of the four lines follow Mendelian pattern of 3∶1 in the inheritance of one pair of genes. The foreign gene can be inherited stably through resistance screening by generation. Analyses of fiber quality trait show that the main function of rabbit keratin gene is enhancement of fiber strength. Fiber strength of all the four transgenic lines increases significantly compared with the control, with incremental ratio of the three lines surpassing 10%.更多还原