【摘要】 调查了广东省东莞市2008年犬的饲养情况及2006年的免疫情况;应用ELISA对2006~2007年采自东莞市32个镇区的1772份犬血清样品进行了狂犬病病毒抗体效价的检测;采用RT-PCR方法对2006~2007年采自上述区域的104份犬脑组织样品和789份犬唾液样品进行了病原学检测。结果,全市共养犬10万多只,其中农村和城镇各占50%,狂犬病疫苗的注射率约为83.5%。1772份犬血清样品中,免疫合格率为52.31%,其中2006年的免疫合格率为35.92%,2007年的免疫合格率为70.69%;893份犬脑组织和唾液样品中,狂犬病病毒的阳性率为1.57%。结果表明,2007年东莞市犬的狂犬病免疫水平有大幅度提高,当地犬可能携带狂犬病病毒。更多还原

【Abstract】 The number and distribution of dogs in Dongguan Prefecture of Guangdong Province were investigated in 2008.Immunization rate against rabies in the prefecture was examined in 2006.1 772 canine sera collected in the prefecture between 2006 and 2007 were examined by ELISA.104 canine brain tissue samples and 789 canine saliva samples were examined by RT-PCR.More than 50 000 dogs are raised in villages,and approximately 50 000 dogs are raised in towns in 2008 in the prefecture.83 500 of 100 000 dogs were injected with rabies vaccines in 2006.Immune eligibility rates of 994 and 778(total 1 772) sera were 35.92% and 70.69% in 2006 and 2007,respectively.Positive rate of rabies antigen was 1.57% in 893 canine brain tissue and saliva samples.The results showed that levels of titer of antibody against rabies in 2007 was significantly higher than that in 2006,and the dogs in the prefecture might carry rabies virus.更多还原