【摘要】 将10周龄雄性昆明小鼠随机分为硝基苯染毒组(26 mg/kg、52 mg/kg、105 mg/kg)、花生油溶剂对照组、生理盐水对照组。对各组试验小鼠进行处理后,于第30 d检测小鼠血清和睾丸中SOD、GSH-Px、CAT的活性及MDA含量,并通过DNA Ladder条带和透射电镜检测睾丸细胞的凋亡。结果,染毒组血清及睾丸中SOD、GSH-Px、CAT的活性显著或极显著(P<0.01或P<0.05)低于溶剂对照组,MDA含量显著或极显著(P<0.01或P<0.05)高于溶剂对照组,染毒组均显示DNA Ladder条带;电镜下细胞核皱缩,异染色质边聚,呈现典型凋亡现象。结果表明,硝基苯中毒能够诱使睾丸发生氧化应激,进而导致睾丸细胞发生凋亡,抗氧化酶在睾丸细胞凋亡中具有一定的作用。更多还原

【Abstract】 Ten week old male Kunming mice were divided randomly into 3 nitrobenzene treated groups(26mg/kg,52mg/kg and 105mg/kg,respectively),arachis oil control group and normal saline control group.The activities of SOD,GSH-Px and CAT and the content of MDA in serum and testicle were exa-mined on day 30 post-treatment.Testis cell apoptosis was detected by DNA Ladder and transmission electron microscope.The activities of SOD,GSH-Px and CAT in the 3 nitrobenzene treated groups were significantly lower than those in the arachis oil control group,and the contents of MDA in the 3 nitrobenzene treated groups were significantly higher than that in the solvent control group(P<0.01 or P<0.05).DNA Ladder appeared in the 3 nitrobenzene treated groups.Typical apoptosis was observed by the electron microscope.The result showed that nitrobenzene could induce oxidative stress,and lead to the apoptosis of testis cell in male mice,and antioxidase played a great role in testis cell apoptosis of the male mice.更多还原