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光照敏感型水稻红根突变体遗传分析及基因的分子定位

Genetic Analysis and Molecular Mapping of Light-Sensitive Red-Root Mutant in Rice(Oryza sativa)

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【作者】 张俊芝刘晓李超肖珂董彦君

【Author】 ZHANG Jun-zhi,LIU Xiao,LI Chao,XIAO Ke,DONG Yan-jun(Laboratory of Plant Genetics and Functional Genes,College of Life and Environment Sciences,Shanghai Normal University,Shanghai 200234,China)

【机构】 上海师范大学生命与环境科学学院植物遗传与功能基因研究室

【摘要】 在自然光条件下水培籼稻品种Nankinkodo中,发现根为红色的自然突变体,命名为HG1。水培条件下该突变体在光照强度大于29μmol/(m2.s)的可见光下,根开始转红,在光照强度为180μmol/(m2.s)的可见光下,呈鲜红,具有明显的光照敏感性。遗传分析表明,该突变体光照敏感性的红根性状由1对显性基因控制,暂命名为Lsr。利用微卫星标记将Lsr基因定位在第4染色体上RM252与RM303之间,遗传距离分别为9.8c M和6.4c M,这为Lsr基因的精细定位和克隆奠定了基础。

【Abstract】 The light-sensitive red-root mutant,designated as HG1,was newly observed in an indica rice variety,Nankinkodo,when the roots were exposed to natural light under hydro-culture conditions.The root color of the mutant began to turn slight-red when the roots were exposed to the light at the intensity of 29 μmol/(m2·s),and turned dark-red at 180 μmol/(m2· s),which suggested that the root color of the mutant was very sensitive to light.Furthermore,genetic analyses showed that the character of light-sensitive red-root in the HG1 mutant was controlled by a single dominant gene,tentatively designated as Lsr.With the SSR markers,Lsr gene was mapped between RM252 and RM303 on chromosome 4 with the genetic distances of 9.8 cM and 6.4 cM,respectively.The results obtained from the study could be useful for fine mapping and cloning of Lsr gene in rice.

【基金】 上海市教育委员会资助项目(06ZZ21);上海市科学技术委员会资助项目(06PJ14074,075405117);国家948计划重大项目(2006-G1);国家航天育种工程资助项目(2006HT100013)
  • 【文献出处】 中国水稻科学 ,Chinese Journal of Rice Science , 编辑部邮箱 ,2008年06期
  • 【分类号】S511
  • 【被引频次】2
  • 【下载频次】85
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