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稻米粒形和垩白度的QTL定位和上位性分析
QTLs Mapping and Epistasis Analysis for Grain Shape and Chalkiness Degree of Rice
【摘要】 利用由181个家系组成的Lemont/特青籼粳交重组自交系群体,以及由161个RFLP、SSR标记和3个形态标记构建的全长为1916.5cM、覆盖水稻基因组12条染色体的连锁图,采用线性模型的复合区间作图方法(QTLMapper V1.0),对粒长、粒宽、长宽比和垩白度等4个稻米品质性状的数量性状座位(QTL)进行了分析。在水稻的所有12条染色体上共定位到7个加性主效QTL和19对上位性QTL,其中控制粒长、粒宽、长宽比的主效QTL各2个,控制垩白度的QTL 1个,分别解释12.8%、40.0%、26.0%和42.1%的表型变异;共检测到6对影响垩白度、6对影响粒长、7对影响长宽比的上位性QTL,分别解释52.2%、31.3%和38.2%的表型变异。结果表明,上位性QTL和主效QTL一样在稻米粒形和垩白度的遗传中起着重要的作用。
【Abstract】 A total of 181 recombinant inbred lines derived from an inter-subspecific cross of Lemont(japonica)/Teqing(indica) and its genetic map were used to identify quantitative trait locus(QTL) controlling rice quality traits such as grain length(GL),grain width(GW),grain length to width ratio(LWR) and chalkiness degree(CD) by a mixed model approach(QTLMapper V1.0).A total of seven QTLs with significant additive effects and 19 pairs of QTLs showing significant additive×additive epistatic effects covering all twelve rice chromosomes were identified.Seven main effect QTLs were detected for GL,GW,LWR and CD,and the percentages of phenotypic variance explained were 12.8%,40.0%,26.0% and 42.1%,respectively.Six,six and seven pairs of epistatic loci were associated with GL,LWR and CD,and the percentages of phenotypic variance explained were 52.2%,31.3% and 38.2%,respectively.The results indicated that the epistatic effects played an important role on the inheritance about grain shape and chalkiness degree in rice.
【Key words】 rice; grain shape; chalkiness degree; quantitative trait locus; epistasis;
- 【文献出处】 中国水稻科学 ,Chinese Journal of Rice Science , 编辑部邮箱 ,2008年03期
- 【分类号】S511
- 【被引频次】45
- 【下载频次】417