【摘要】 对经EDTA和盐酸预处理后提取得到的天然胶原的物理化学性能进行了表征。经盐酸预处理后提取得到的胶原(HPC)的等电点(5.38±0.02),低于经EDTA预处理后提取得到的胶原(EPC)的等电点(5.87±0.03),HPC的自由氨基含量低于EPC;同时,HPC的平均粒径和相对黏度均大于EPC。两者的氨基酸组成相似,与商品小牛皮胶原(CCC)差别不大;通过电泳、圆二色谱分析分别验证了EPC和HPC的多肽链和三股螺旋结构的完整性。差式扫描量热法分析表明:EPC和HPC与商品小牛皮胶原一样,具有较好的热稳定性。更多还原

【Abstract】 The physicochemical properties of collagen extracted from EDTA-and HCl-pretreated bovine limed split wastes respectively were charactered.The isoelectric point of HPC(5.38±0.02) is lower than that of EPC(5.87±0.03).And the primary amino acid content of HPC is lower than that of EPC.Simultaneity,HPC has larger Z-particle size and higher relatively viscosity than those of EPC.The amino acid compositions of EPC and HPC are similar to each other,although there is a little difference compared to that of commercial calfskin collagen(CCC).The results of sodium dodecyl sulphate polyacrylamide gel electrophoresis analysis(SDS-PAGE) and circular dichroism(CD) spectras represented the integralities of their polypeptide and triple helix conformations.Thermal behaviors of EPC and HPC,reviewed using differential scanning calorimetry(DSC),are almost as same as that of CCC.更多还原