【摘要】 目的:评价莱菔硫烷(SFN)与5-氟尿嘧啶(5-Fu)对人高转移唾液腺腺样囊性癌细胞(ACC-M)生长的抑制作用,同时检测参与诱导抗癌药物耐药性的核因子κB(NF-κB)的表达。方法:中位效应法评价SFN与5-Fu联用对ACC-M细胞增殖的影响,NF-κB p65表达采用Western印迹法检测。采用SPSS10.0软件包对所得数据进行t检验和方差分析。结果:SFN与5-Fu单独作用于ACC-M时的中位抑制浓度分别为(38.58±4.97)μmol/L和(120.90±12.96)μmol/L。两者联合使用时,对ACC-M细胞生长呈现协同抑制作用,并且引起细胞核NF-κB p65表达显著降低。与对照组相比,两药联合使用时,NF-κB p65的表达约降低5倍,P<0.05。结论:SFN与5-Fu联合使用可协同抑制ACC-M细胞增殖,该作用与SFN、5-Fu协同抑制细胞核NF-κB p65的表达有关。更多还原

【Abstract】 PURPOSE:To evaluate the growth-inhibitory effect of sulforaphane (SFN) and 5-fluorouracil (5-Fu) against the proliferation of human high metastatic cell line of salivary gland adenoid cystic carcinoma (ACC-M). The expression of nuclear factor kappa B (NF-κB) inducing resistance to anticancer chemotherapeutic agent was also detected. METHODS: The combination effect of SFN and 5-Fu was quantitatively determined using the method of median effect principle and the combination index. The NF-κB p65 expression after treatment with the SFN-5-Fu combination was also evaluated by Western blotting analysis. Statistical comparison was performed by Student’s t test and analysis of variance (ANOVA), using SPSS 10.0 Software Package. RESULTS: The IC50s for SFN and 5-Fu in ACC-M cells were 38.58±4.97 μmol/L and 120.90±12.96 μmol/L, respectively. Treatment of ACCs cells with SFN and 5-Fu in combination, led to synergistic inhibition on cell growth and a decreased expression of NF-κB p65 protein. When the two drugs were combined, the level of NF-κB p65 protein expression was decreased almost 5-fold (P<0.05). CONCLUSION: The results demonstrate synergism between SFN and 5-Fu against the proliferation of ACC-M cells, which is associated with the decreased expression of NF-κB p65 protein.更多还原