【摘要】 目的:观察神经生长因子(NGF)对实验性急性颅脑损伤后大鼠神经病学评分和脑组织中一氧化氮合成酶(NOS)含量的影响,并探讨其作用机制。方法:①将27只急性颅脑损伤大鼠随机分为3组,分别用NGF、胞磷胆碱钠(CS)和生理盐水(NS)治疗,于治疗前后检查动物的神经病学评分改变。②再将65只急性颅脑损伤大鼠随机分为3组,即实验组(30只)、对照组(30只)和正常组(5只)。实验组急性颅脑损伤后即刻肌肉注射神经生长因子,对照组则注射等量生理盐水。对照组和实验组大鼠分别在脑损伤后1h、3h、6h、8h、24h断头取脑,对大鼠急性颅脑损伤后脑组织中NOS含量进行检测。结果:①与治疗前比较,NGF和CS组治疗后神经病学评分均降低(P<0.05);与盐水对照组比较,NGF和CS组治疗后神经病学评分均降低(P<0.05)。②大鼠脑皮质中NOS活性在伤后1h、3h较正常组显著升高(P<0.01)。神经生长因子实验组与盐水对照组比较,NOS含量在脑损伤后1h、3h、6hNOS含量明显下降(P<0.05)。结论:NGF可明显促进急性脑损伤大鼠神经功能的恢复;NGF可通过抑制急性脑损伤后NOS的升高,抑制了一氧化氮的毒性作用,从而保护了脑神经元。更多还原

【Abstract】 Objective: To study the curative effects of nerve growth factor(NGF) by changes of neurological grades and nitric oxide synthetase(NOS) level in experimental acute cerebral injury rats. Method:①Twenty seven rat models were randomly divided into three groups which were respectively treated with NGF,Citicoline Sodium(CS) or normal saline (NS) for 20 days,and the neurological grades of rat models were observed before and after above treatments. ②Sixty five rats of acute cerebral injury were divided into three groups: treated group (30 rats,treated with nerve growth factor),control group(30 rats,treated with normal saline) and normal group(5 rats,acute cerebral injury rats),the levels of NOS were measured at the 1st、3rd、6th、8th、24th hour after acute cerebral injury. Result:①The neurological grades of both NGF and CS treated groups were highly lowered compared with that of pretreated groups and NS control group (both P<0.05). ②NOS level of cerebral injury area was higher than that in control group at the 1st hour、3rd hour after acute cerebral injury. NOS level in nerve growth factor treatment group were obviously lower than that in control group post-traumatic at the 1st、3th and 6th hour (both P<0.05). Conclusion: Nerve growth factor can protect cerebral against injury in vivo and accelerate the nervous function recovery of the rat models with acute cerebral injury. One of the mechanisms may be the nerve growth factor prohibits neurotoxicity of nitric oxide.更多还原