【摘要】 为研究钙稳态失衡在LaCl3诱导MDCC-MSB1细胞凋亡中的作用,将MDCC-MSB1细胞常规培养于RPMI-1640培养液中,加入终浓度为0.5、1.0、1.5、2.0、2.5、3.0、3.5和4.0mmol/L的LaCl3,继续培养24h后,应用MTT法检测细胞增殖抑制率,DNALadder法和TUNEL法检测细胞凋亡,以Fura-2为荧光探针检测细胞内[Ca2+]i的变化。在LaCl3浓度为0.5～4.0mmol/L范围内,细胞的增殖抑制率增加,细胞凋亡数量和细胞内[Ca2+]i呈升高趋势,并呈剂量-效应关系。结果表明,LaCl3能抑制MDCC-MSB1细胞的增殖,并可能通过改变[Ca2+]i而诱导其发生凋亡。更多还原

【Abstract】 To study the effect of the disequilibrium of calcium homeostasis on the apoptosis of MDCC-MSB1 cells induced by lanthanum chloride,MDCC-MSB1 cells was conventionally cultured in RPMI-1640 for 24 h,which was added with concentration are 0.5,1.0,1.5,2.0,2.5,3.0,3.5 and 4.0 mmol/L.to utilize MTT to detect the cell multiplication inhibition ratio,use DNA Ladder and TUNEL to detect apoptosis,use the Fura-2/AM as the probe to detect the calcium ion within nerve cells concentration([Ca2+]i).LaCl3 concentration is in the confine of 0.5 to 4.0 mmol/L,cell multiplication inhibition ratio was increasing,apoptosis and [Ca2+]i were offering an increasing tendency,which present dose-effectiveness relationship.The results showed that LaCl3 could restrain the proliferation of MDCC-MSB1 cells and derive apoptosis happens by changing [Ca2+]i.更多还原