【摘要】 采用斑马鱼胚胎发育技术,探讨了人工纳米材料富勒烯(C60)的1种水溶性衍生物[C60(OH)16-18]和3种水悬浮液(nC60)对斑马鱼胚胎的发育毒性.这3种nC60分别是nC60/TTA,nC60/甲苯和nC60/aq.结果表明,38mg/LnC60/甲苯、5.0mg/LnC60/aq和50mg/LC60(OH)16-18均未对斑马鱼胚胎发育产生毒性,而1.5mg/LnC60/TTA不仅能导致胚胎或幼鱼发育的延迟,存活率和孵化率的下降,甚至能造成部分斑马鱼心包囊水肿和畸形.C60,nC60的制备过程以及nC60本身的结构均能影响其潜在生物毒性.更多还原

【Abstract】 The developmental toxicity of water-soluble C60 aggregates (nC60) produced through three methods and a hydroxylated C60 derivative (fullerol, C60(OH)16-18) were compared using a zebrafish (Danio rerio) 4-day embryo-larval bioassay. The three methods were solvent exchange using toluene, acetone and THF (nC60/TTA), mixing C60 in a toluene solution with water through sonication (nC60/toluene) and extended mixing of C60 in water without the use of organic solvents (nC60/aq). Within 96h of exposure, nC60/toluene at 38 mg/L, fullerol at 50 mg/L and nC60/aq at 5 mg/L did not show any toxicity to zebrafish embryos. In contrast, nC60/TTA at 1.5 mg/L delayed zebrafish embryo and larva development, decreased its livability and hatching rate, and caused pericardial edema. The prepare methods of nC60 as well as the structure of nC60 itself could affect the potential bio-toxicity of nC60.更多还原