【摘要】 背景 WNK1是新近发现的肾脏多个离子转运体及通道的调控蛋白,与醛固酮离子转运途径有关。目的探讨高钾饮食致体内醛固酮增高对大鼠肾脏 WNK1不同转录本表达的作用及其意义。方法采用高钾饮水饲养 Wistar 大鼠8只,检测血清、24 h 尿液离子质量浓度及血清醛固酮质量浓度。半定量 RT-PCR 方法检测大鼠肾脏 WNK1-L和 WNK1-KS 基因表达情况。结果与正常对照组(n=8)比较,高钾组(n=8)血清醛固酮水平显著升高[(1.86±0.66)比对照组:(0.25±0.04)μg/L,P<0.01],为对照的7.4倍。血清及尿液钾离子和尿液氯离子质量浓度升高(P<0.05),尿液钠离子质量浓度下降(P<0.05),血清钠离子和氯离子无差异(P>0.05)。高钾组WNK1-L 表达量无差异(0.27±0.07比0.26±0.07,P>0.05),WNK1-KS 表达量显著上升(1.60±0.22比0.38±0.08,P<0.01)。结论高钾饮食导致大鼠醛固酮质量浓度升高,醛固酮可通过调控 WNK1-KS 表达起到排钾保钠的生理作用。更多还原

【Abstract】 Background WNK1 is a recently found upstream kinase of multiple transporters and channels in kidney,while might be involved in the biological effect of aldosterone.Objective To investigate the effect of in- creases in aldosterone induced by high dietary potassium intake on the expression of rat kidney WNK1 isoforms. Methods High KCl solution(0.5 mol/L)in diet was administrated to Wistar rats(n=8).The concentrations of e- lectrolytes and aldosterone in serum as well as 24 hour electrolytes concentrations in urine were measured.RT-PCR was used to detected the expression of rat kidney WNK1 isoforms.Results Serum concentrations of aldosterone in high K+diet rats was 7.4 times of that in the control group(1.86±0.66 vs control:0.25+0.04 μg/L,P<0.01). Serum K~+and urine K~+and Cl~-in the experimental rats were significant higher than those in normal rats while the urine Na+was lower(P<0.05).There were no significant difference of serum Na~+or Cl~-between two groups(P >0.05).The expression of WNK1-KS is higher in the experimental rats than that in control rats(1.60±0.22 vs 0.38±0.08,P<0.01)while no differences was found in the expression of WNK1-Lin both groups(0.27±0.07 vs 0.26±0.07,P>0.05).Conclusion The high dietary potassium intake induces the secretion of aldosterone.Al- dosterone regulating serum and urine sodium and potassium is associated with upregulated expression of WNK1-KS.更多还原