【摘要】 目的:探讨prostasin基因在卵巢癌组织中的表达及意义。方法:①应用4 097靶基因点基因芯片对5例卵巢浆液性腺癌及5例正常卵巢组织进行基因表达谱分析,筛查差异表达基因。②应用RT-PCR检测10例卵巢癌组织、10例卵巢瘤组织及10例正常卵巢组织中prostasin mRNA的表达。结果:①基因芯片筛查出卵巢浆液性腺癌差异表达基因58个,其中表达增高(上调趋势)基因30个,表达降低(下调趋势)基因28个;prostasin基因为表达上调基因(cy5/cy3=3.018)。②prostasin基因在卵巢癌组织中表达显著增高(prostasin/actin:0.415±0.177),与正常卵巢组(prostasin/actin:0.192±40.128)及卵巢瘤组(prostasin/actin:0.237±0.232)比较无显著性差异(P>0.05)。结论:基因芯片可以用于筛查卵巢癌差异表达基因,prosta-sin为表达上调基因,prostasin mRNA在卵巢癌组织中表达显著高于在正常卵巢及卵巢瘤组织中表达。更多还原

【Abstract】 Objective:To study the expression of prostasin in ovary cancer and its significances.Methods:①Gene microarray containing 4 097 genes was used to analyze gene expression patterns in tissue samples from 5 cases of human ovarian serous cystadenocarinoma and 5 cases of normal ovarian.②RT-PCR method was used to investigate the expression of prostasin mRNA in tissues samples of 10 eases ovarian cancer,10 cases benign ovarian tumor and 10 eases normal ovarian.Results:①58 genes of differential expression were found in ovarian cancer,the expression of 30 genes increased(up-regulated),the expression of 28 genes decreased(down-regulated),prostasin was the up-regulated gene(Cy5/Cy3=3.018).②The level of prostasin mRNA expression in ovary cancer tissues(prostasin/actin: 0.415±0.177) was significantly higher than that in normal ovary tissues(prostasin/actin:0.192±0.128) and behign ovary tumor tissues(prostasin/actin: 0.237±0.232)(P<0.05).Conclusion:Gene microarray could screen differential expression gene in ovary cancer;prostasin is up-regulated gene;the expression of prostasin mRNA is significantly higher than that of normal and benign ovary tumor tissues.更多还原