【摘要】 目的:探讨参附注射液对新生大鼠缺氧缺血性脑损伤(HIBD)后海马神经元Bax、Bcl-2蛋白表达的影响及其治疗作用。方法:建立新生大鼠HIBD模型,把实验分成两部分:①观察缺氧缺血后3、7、14、28天各组的体重变化以及各组28天内生存率;②观察缺氧缺血前2h,缺氧缺血后2、12、24h,3、7、14、28天各时间点新生大鼠海马CA1区神经元Bax、Bcl-2蛋白的表达。每组实验随机分为4组:假手术组(S组)、生理盐水对照组(C组)、参附预处理组(P组)、参附治疗组(SF组)。结果:SF组和P组较C组Bax表达明显下调(P<0.05,P<0.01),而Bcl-2表达显著上调(P<0.05,P<0.01),生长发育和生存情况明显好于C组。结论:参附改善了新生大鼠HIBD后的生长发育,提高了其生存率;可明显下调新生大鼠HIBD后海马神经元Bax蛋白表达及上调Bcl-2蛋白的表达。更多还原

【Abstract】 Objective:To observe the effect on the expression of Bax and Bcl-2 of neurons in hippocampal CA1 region of neonatal rat with HIBD,Shenfu injection was used to treat HIBD.Methods:The experiment included two parts,one was to observe the effect on the physical development and survival rate,the other was to investigate the expression of Bax and Bcl-2 of neurons in left hippocampal CA1 region..Models of postnatal 7-day Sprague Dawley(SD)rats with HIBD were established.The neonatal rat with HIBD were equally divided into 4 groups:sham operation(S),control group(C),Shenfu injection pretreatment(P),and Shenfu injection treatment(SF).The increasing body weight of rats in every group was observed at 3,7,14 and 28 d after HI insult,and the survival rate of rats in every group on 28 d after HI insult.Similarly,four groups were established,however they were further divided into several subgroups again according to the time point 2 h before and 2,12,24 h,3,7,14 and 28 d after HI insult(n=6).After treatment with Shenfu injection,the expression of Bax and Bcl-2 in left hippocampal CA1 region at different time was investigated.The expression of Bax and Bcl-2 was performed by immunohistochemistry.Results:The increase of body weight in group C at 3,7,14 and 28 d after HI insult were significantly less than those of group S(P<0.01)and also were group P and SF(P<0.01,P<0.05).The increase of body weight in group P at 3,7,14 and 28 d after HI insult were significantly higher than those of group C(P<0.01,P<0.05),and the increase of body weight in group SF at 7,14 and 28 d after HI insult were significantly higher than those of group C(P<0.05).The survival rate of group C(60%)was significantly less than that of other groups(P<0.05),and there was no obvious difference in survival rates between other groups(P>0.05).The increasing expression of Bax and Bcl-2 in hippocampal CA1 region in group C were observed at 2 h after insult,and at 12 h after insult,compared to group S,the expression of Bax or Bcl-2 in group P was markedly lower or higher than that of group C(P<0.01).The degree of expression were reached the peak at 1 d after insult,and the expression of Bax or Bcl-2 in group P was still markedly lower or higher than that of group C(P<0.01).At 3 d after HI the expression of Bax and Bcl-2 was gradually decreasing,but the expression of Bax or Bcl-2 in group P and SF was markedly lower or higher than that of group C(P<0.01).At 7 d after HI,the expression of Bax and Bcl-2 was decreased markedly,but the expression of Bax or Bcl-2 in group C was markedly higher or lower than that of group P and group SF(P<0.01).Conclusion:Shenfu injection could enhance the physical development and elevate the survival rate in neonatal rats with HI insult.It significantly prevents apoptosis of hippocampus neurons from HI insult by down-regulation of the pro-apoptotic gene Bax,otherwise,and up-regulation of the anti-apoptotic gene Bcl-2.更多还原