【摘要】 目的:建立简单快速的RP-HPLC方法测定猫豆中左旋多巴,考察白花和紫花猫豆种子、茎、叶、花、豆荚、根中左旋多巴的含量及其不同时期的变化,以确定猫豆的适宜药用部位、最佳采收期和栽培品种。方法:采用反相高效液相法,色谱柱:瓦里安公司Polaris C18柱(250 mm×4.6 mm,5μm);流动相:甲醇:0.01 mol/L磷酸二氢钾-磷酸缓冲液(10∶90,pH3.38)用于分析种子、花、叶、茎、根中L-Dopa含量,甲醇:0.01 mol/L磷酸二氢钾-磷酸缓冲液(5∶95,pH3.17)用于分析豆荚中L-Dopa含量;流速:1 mL/min;检测波长:280 nm。结果:猫豆种子中左旋多巴含量最高,其在荚果成熟并开始干燥时含量最高,白花猫豆左旋多巴含量比紫花高。结论:建议猫豆采收期为荚果成熟开始干燥时;栽培品种为白花猫豆。更多还原

【Abstract】 AIM:To establish a simple RP-HPLC method for determining levodopa in Mucuna pruriens var.utilis,and study the levodopa content in different parts of Mucuna pruriens var.utilis and in various growth periods two cultispecies(white flower and purpleflower). METHODS: A RP-HPLC method was used for the determination of levodopa on a Polaris RP-C18(250 mm×4.6 mm,5 μm) column.The mobile phase was methanol-potassium dihydrogne phosphate(10∶90,pH3.38)for seed,flower,leaf,stem and root,and methanol-potassium dihydrogne phosphate(5∶95,pH3.17)for pod.The detection wavelength was at 280 nm,and flow rate was 1 mL/min. RESULTS: The levodopa content in seed and white-flower cultispecies were higher.In the growth periods,the levodopa content was higher in the sample collected when the pod matured and became dry. CONCLUSION: White-flower cultispecies of M.pruriens var.utilis is the good cultispecies.matured and dry pod is the optimal harvesting time for this herbal medicine.更多还原