【摘要】 目的:测定桃红四物汤水提液(桃仁、红花等)中没食子酸、芍药苷和红花黄色素的含量。方法:没食子酸、芍药苷和红花黄色素含量分析采用高效液相色谱法。没食子酸色谱条件:流动相:甲醇-磷酸水溶液(pH=3.0)(5∶95),柱温35℃,检测波长:271 nm。红花黄色素色谱条件:流动相:甲醇-磷酸水溶液(pH=3.0)(80∶20),柱温25℃,检测波长:403nm。芍药苷色谱条件:流动相:甲醇-磷酸二氢钾溶液(38∶62),检测波长:230 nm,柱温:25°C。流速均为1.0 mL/min,进样量均为:20μL。结果:HPLC测定没食子酸在4.547×10-3~1.455×10-1μg/μL(r=0.9993)范围内线性关系良好,平均回收率为98.0%,RSD为1.8%。芍药苷在0.010~0.200μg/μL(r=0.999 8)范围内线性关系良好,平均回收率为98.4%,RSD为0.69%。红花黄色素在2.34×10-2~7.50×10-1μg/μL(r=0.9994)范围内线性关系良好,平均回收率为99.2%,RSD为0.88%。结论:本法简单快捷,重现性好,可作为桃红四物汤中没食子酸、芍药苷和红花黄色素的质控方法。更多还原

【Abstract】 AIM: To determine gallic acid,paeoniflorin and safflor yellow in Taohong Siwu Decoction（Semen Persicae,Flos Carthamietc.） METHODS: To analyse three constituents by HPLC on a Hypersil BDS C₁₈ column and gallic acid detection wavelength was set at 271 nm.The mobile phase was methanol-water（5∶95） （adjusted to pH=3.0 with H₃PO₄） at a flow rate of 1.0 mL/min. Safflor yellow detection wavelength was set at 403 nm.The mobile phase was methanol-water（80∶20） （adjusted to pH=3.0 with H₃PO₄） at a flow rate of 1.0 mL/min. Paeoniflorin detection wavelength was set at 230 nm.The mobile phase was methanol-KH₂PO₄ at a flow rate of 1.0 mL/min. RESULTS: Gallic acid showed a good linear relationship at the range of 4.547×10^-3-1.455×10^-1 μg/μL（r=0.999 3）.Paeoniflorin showed a good linear relationship at the range of 0.010-0.200 μg/μL（r=（0.999 8）） and safflor yellow showed a good linear relationship at the range of 2.34×10^-2-7.50×10^-1 μg/μL（r=（0.999 4））.The average recoveries were 98.0% for gallic acid,98.4% for paeoniflorin and 99.2% for safflor yellow.The RSD of the method were 1.8%,0.69% and 0.88%,respectively. CONCLUSION: The method is proved to be fast,feasible,simple,and can be used for quality control of gallic acid,paeoniflorin and safflor yellow in Taohong Siwu Decoction.更多还原