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黄芩总黄酮的分离纯化工艺研究
Separation and purification process of total flavonoids from Scutelaria baicalensis
【摘要】 目的:考察10种型号的大孔吸附树脂对黄芩总黄酮的吸附性,优选出分离黄芩苷、汉黄芩苷、黄芩素、汉黄芩素的大孔吸附树脂。方法:用HPLC法,分别以黄芩苷、汉黄芩苷、黄芩素、汉黄芩素的含量为考察指标,采用静态吸附和动态吸附相结合的方法进行筛选。结果:树脂型号不同,上样条件不同,对黄芩中4种黄酮类化合物的吸附力和解析率也不同。结论:综合考虑静态吸附和动态吸附的结果,以SP-825为最佳。
【Abstract】 AIM:To review the 10 kinds of macroporous resin’s absorption of total flavnoids to screen out the one which could separate the Baicalin,Baicalein,Wogonoside,Wogonin simultaneously. METHODS: By HPLC,using the content of Baicalin,Baicalein,Wogonoside,wogonin as the marker,adopting a static adsorbtion in combination with dynamic state. RESULTS: The difference in adsorption and desorption among macroporous resins and adsorption condition arose from four kinds of flavonoids. CONCLUSION: At a comprehensive estimate SP-825 resin was regarded as the ideal for adsorption and desorption.
【Key words】 macroporous adsorption resin； total flavonoids； static adsorption； dynamic adsorbtion；
- 【文献出处】 中成药 ,Chinese Traditional Patent Medicine , 编辑部邮箱 ,2008年02期
- 【分类号】R284
- 【被引频次】13
- 【下载频次】918