【摘要】 目的:观察兔股动脉粥样硬化斑块发展过程中斑块内组织因子(TF)及组织因子途经抑制物-1(TFPI-1)、组织因子途经抑制物-2(TFPI-2)的变化。方法:新西兰雄兔通过高脂饲养合并股动脉球囊损伤建立动脉粥样硬化斑块模型。在建模8周、10周和12周,分别截取球囊损伤处股动脉标本,检测斑块组织内TF、TF-PI-1和TFPI-2的蛋白表达;并测定斑块内TF、TFPI-1和TFPI-2的mRNA水平。结果:自8周、10周到12周,血管斑块中TF、TFPI-1和TFPI-2的观测区域内阳性染色面积均逐渐增加。自8周、10周到12周,血管斑块中TF的mRNA水平和TFPI-1的mRNA水平均逐渐增加;TFPI-2mRNA表现为前期无明显变化,12周时显著下调。结论:在兔股动脉粥样硬化斑块的形成过程中,随着斑块内TF基因及蛋白表达的增加,TFPI-1和TFPI-2反应性表达增加,但其表达水平未能完全抑制斑块的进展。更多还原

【Abstract】 AIM: To observe the regulatory changes of tissue factor(TF),tissue factor pathway inhibitor-1(TFPI-1) and tissue factor pathway inhibitor-2(TFPI-2) associated with the formation of atherosclerotic plaque on femoral artery of New Zealand rabbits.METHODS: New Zealand rabbits were fed with high cholesterol diet and received femoral artery balloon injury to establish moderated atherosclerotic plaque animal model.These rabbits were classified into 8 weeks group,10 weeks group and 12 weeks group.The expressions of factor TF,TFPI-1 and TFPI-2 in femoral artery were detected by immunohistochemical methods at 8th,10th and 12th week.The positive areas of the slice were analyzed by semi-quantification system.Fluorescence quantification PCR(FQ-PCR) was used to detect the copies of mRNA of TF,TFPI-1 and TFPI-2 in each group.RESULTS: The expressions of TF,TFPI-1 and TFPI-2 determined by immunohistochemical methods were increased from 8th,10th to 12th week,respectively.In each group,the quantity of mRNA of TF and TFPI-1 at the 8th,10th and 12th week was soared,respectively.In each group,the quantity of mRNA of TFPI-2 did not change in early phase,but decreased at 12th week.CONCLUSION: In the process of the atherosclerotic plaque formation in rabbit femoral artery model,the expressions of TFPI-1 and TFPI-2 proteins are increased.However,the expression of TPFI-1 or TFPI-2 could not stave off the development of plaque formation.更多还原