【摘要】 目的:观察甘氨酸脂质体对缺氧/复氧损伤心肌细胞线粒体膜电位及凋亡的影响。方法:建立培养乳鼠心肌细胞缺氧/复氧(H/R)损伤模型,以DiOC6(3)为荧光分子探针检测实验各组心肌细胞线粒体膜电位;以AnnexinV联合PI染色法检测实验各组心肌细胞凋亡率。结果:(1)H/R处理组心肌细胞线粒体膜电位明显低于对照组(P<0.01),甘氨酸脂质体处理组心肌细胞线粒体膜电位降低最少,弱荧光部分细胞百分率为(9.61±0.76)%。与甘氨酸组比较有显著差异(P<0.01)。(2)H/R处理组心肌细胞凋亡率为(20.78±1.58)%,明显高于对照组(P<0.01)。甘氨酸脂质体处理组心肌细胞凋亡发生率低于甘氨酸组(P<0.01)。空白脂质体组细胞凋亡发生率与H/R组比较差异无统计学意义(P>0.05)。结论:甘氨酸脂质体能抑制培养心肌细胞缺氧/复氧损伤诱导的心肌细胞线粒体膜电位下降和心肌细胞凋亡,脂质体携载甘氨酸能更好发挥其细胞保护作用。更多还原

【Abstract】 AIM:To observe the effect of glycine liposomes on the mitochondrial membrane potential and the apoptosis rate in cardiomyocytes induced by hypoxia/reoxygenation injury.METHODS:A cardiomyocyte injury model was established by using hypoxia/reoxygenation.DiOC6(3)as fluorescence molecular probe was used to detect the mitochondrial membrane potential in each group.The method of Annexin V associated with PI was used to detect the apoptosis ratio in each group.RESULTS:(1)The result of flow cytometry showed that the mitochondrial membrane potential of cardiomyocytes in H/R group was obviously lower than that in control group(P<0.01).The decrease in mitochondrial membrane potential in Gly-liposome group was the lowest,the percentage of cells about the part of hypofluorescence was(9.61±0.76)%,which was lower than that in glycine group(P<0.01).(2)The apoptosis rate of cardiomyocytes in H/R group was higher than that in control group(20.78±1.58)%,P<0.01.After the treatment of Gly-liposome,the apoptosis rate of cardiomyocytes was lower than that in glycine group(P<0.01).No difference in the apoptosis ratios between blank-liposome group and H/R group was observed(P>0.05).CONCLUSION:Glycine liposomes protect cultured cardiomyocytes against hypoxia/reoxygenation injury.Glycine liposomes produce the better protective effects than glycine.更多还原