【摘要】 目的:通过研究卵巢癌细胞对CD8+ T细胞ζ链表达及分泌Tc1/Tc2型细胞因子影响,探讨其在卵巢癌免疫抑制中的作用。方法:Western印迹方法检测3株卵巢癌细胞(OVCAR3、CAOV3和SKOV3)培养上清液作用后CD8+ T细胞ζ链蛋白的表达;分别采用MTT、逆转录-聚合酶链反应(RT-PCR)方法观察卵巢癌细胞培养上清液对CD8+ T细胞增殖和分泌Tc1/Tc2型细胞因子的影响。结果:(1)与对照组比较,OVCAR3、CAOV3和SK-OV3细胞培养上清液均能抑制CD8+ T细胞中ζ链的表达,ζ链蛋白平均吸光度值分别为(0.346±0.004;0.349±0.007;0.387±0.011;0.616±0.013);(2)3株卵巢癌细胞培养上清液皆抑制CD8+ T细胞增殖,分泌的Tc1细胞因子IFN-γ显著降低,而Tc2细胞因子IL-10显著增高。结论:卵巢癌细胞源性的免疫抑制因子通过下调CD8+ T细胞ζ链的表达,从而抑制其增殖并导致Tc1/Tc2细胞因子分泌失衡,这可能是卵巢癌诱导腹腔免疫抑制的机制之一。更多还原

【Abstract】 AIM:To investigate the effect of ovarian carcinoma cells on ζ chain expression and the secretion of Tc1/Tc2 type cytokine in CD8+ T cells,and its role in the ovarian carcinoma induced immunosuppression.METHODS:The supernatants of human ovarian carcinoma cell lines of OVCAR3,CAOV3 and SKOV3 and RPMI-1640 were added into CD8+ T cells(groups I,II,III,and control),which were isolated from the peripheral venous blood of healthy persons.The expression of ζ chain was analyzed by Western blotting.Thiazolyl blue(MTT)method was used to detect the effects of those cell line supernatants on the growth of CD8+ T cells.The secretion of the Tc1 type cytokine interferon(IFN)-γ mRNA and the Tc2 type cytokine interferon(IL)-10 mRNA were detected by RT-PCR.RESULTS:The expression of ζ chain was significantly lower in groups I,II,and III in comparison with that in control group.The absorbance at the wavelength 570 nm of CD8+ T cells culture in the group I,II,and III was all significantly lower than that in the control group.The IFN-γ expression was significantly lower in groups I,II,and III in comparison with that in control group,while the expression of IL-10 was significantly higher.CONCLUSION:Ovarian carcinoma may suppress CD8+ T cell proliferation and secretion of the Tc1/Tc2 type cytokine through inhibition of ζ chain,which may play an important role in the ovarian carcinoma induced immunosuppression.更多还原