【摘要】 目的建立一种测定构树叶辅酶Q10含量的方法。方法构树叶用75%乙醇提取,萃取,蒸馏,浓缩。色谱柱为C18250mm×4.6mm i.d10μm,流动相为无水乙醇-甲醇(65:35),流速为1ml/min,检测器波长275nm。结果辅酶Q10在4～64μg/ml(r=0.9997)范围内具有良好的线性关系。平均加样回收率为98.66%,RSD为0.97%(n=6)。构树叶中辅酶Q10的最低检测限为10ng/ml(S/N=3)。结论该法快速,简便,准确。适宜于构树叶辅酶Q10含量的检测。更多还原

【Abstract】 Objective A simple and rapid high performance liquid chromatographic(HPLC) method for the determination of coenzyme Ql0(CoQ10) in Broussonetia paryifera was developed. Method The sample of Broussonetia paryifera leares was extracted by 75% ethanol,extraction,distillation,concentration. Hypersil C18(250mm× 4.6 mm i.d 10 μm) was selected as the analytical column,ethanol-methanol(65:35 by volume) as the mobile phase with flow-rate of 1 ml/min and UV 275 nm as the detection wavelength. Results The1inear range of the calibration curve of concentration vs. peak height was 4-64 μg/ml with a coefficient of determination was 0.9997. The average recovery rate was 98.66% and the RSD was 0.97%(n=6) . The detection limit of CoQ10 in Broussonetia paryifera was 10ng/ml(S/N=3) . Conclusion This method was rapid,simple and suitable for the determination of CoQl0 in Broussonetia paryifera.更多还原