节点文献

与黄瓜矮生基因连锁的ISSR标记及其SCAR转换

Molecular Tagging of ISSR and Conversion of SCAR Marker Linked to a Mutant Dwarf Gene in Cucumber

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 嵇怡徐强缪旻珉梁国华陈学好

【Author】 JI Yi1,XU Qiang1,MIAO Min-min1,LIANG Guo-hua2,and CHEN Xue-hao1(1School of Horticulture and Plant Protection,Yangzhou University,Yangzhou,Jiangsu 225009,China;2Key Laboratory of Education Ministry for the Plant Function Genomics,Yangzhou University,Yangzhou,Jiangsu 225009,China)

【机构】 扬州大学园艺与植物保护学院扬州大学教育部植物功能基因组重点实验室

【摘要】 以黄瓜矮生品系D8与蔓生品系JIN5杂交并自交得到的124株F2代为试材,应用ISSR分子标记技术和BSA(混合群体分组分析)法寻找与黄瓜矮生基因连锁的分子标记。从80条ISSR引物中筛选到一个黄瓜矮生性状特异的多态性引物UBC818。经F2代单株验证,UBC818的扩增片段在蔓生黄瓜JIN5植株中稳定出现,而在矮生黄瓜D8植株中没有。连锁关系分析表明,标记与黄瓜矮生基因间的遗传距离为11.1cM。根据对该片段的序列分析结果重新设计了1对引物,将ISSR标记成功转化成了SCAR标记,并命名为UBC818-S。

【Abstract】 In this study,124 of F2 plants from the cross combination of the dwarf cucumber D8 and the vine plant JIN5 were used to screen the molecular marker linked to the dwarf mutant gene in cucumber with ISSR molecular tagging and BSA(bulked segregation analysis)method.UBC818,a specific primer of polymorphism,was obtained from eighty ISSR primers screening for a cucumber dwarf traits specific in the dwarf line.The marker from the results of PCR with UBC818 was tested in individual DNAs reaction for the F2 population and the UBC818 amplified fragment could only be detected in the vine plants.Linkage analysis showed that the genetic distance between the specific marker and the cucumber dwarf mutant gene was 11.1 cM.According to the sequence of the fragment analysis,one pair of primers had been designed.The ISSR marker was successfully converted into a SCAR marker which was named UBC818-S.

【关键词】 黄瓜矮生基因ISSRSCAR
【Key words】 cucumberdwarf mutant geneISSRSCAR
【基金】 江苏省农业高技术项目(BG2004313)
  • 【文献出处】 园艺学报 ,Acta Horticulturae Sinica , 编辑部邮箱 ,2008年11期
  • 【分类号】S642.2
  • 【被引频次】28
  • 【下载频次】362
节点文献中: 

本文链接的文献网络图示:

本文的引文网络