【摘要】 介绍一种改良的扩增基因5′cDNA序列的方法(改良TdT加尾扩增法)。以龙眼为材料,采用TdT(末端脱氧核苷酸转移酶)加尾、锚定PCR、巢式PCR、降落PCR等多种策略。通过与常规方法(用TaKaRa试剂盒)对比试验,发现该方法原理简单,操作性强;扩增特异性高,结果真实可靠;同时还具有快速低廉的特点,适合在一般实验室中推广使用。更多还原

【Abstract】 In order to obtain the full-length sequence of genes more efficiently, a modified method of amplifying the 5′ cDNA sequence, named as modified TDT tailing amplified method, was designed. Many strategies were used in this method including TdT tailing, anchored PCR, nested PCR, touchdown PCR. Compared with a regular method using a kit (5′-Full RACE Core Set, TaKaRa), the modified method had many advantages, such as simple principle, easy operability, high amplified specificity, and reliable results. Moreover, it costs less money and time. Therefore, it is worth popularizing in ordinary laboratories.更多还原