【摘要】 利用荧光AFLP标记技术,采用8对EcoR I/Mse I引物对山东省25个石榴品种进行AFLP-PCR分析,结果表明:25个石榴品种扩增的多态性位点数从70条到126条不等,平均90.25条,平均多态性位点41.78%;所有检出位点的平均观测等位基因数、平均有效等位基因数、平均Nei’s基因多样度和平均香农信息指数分别为1.4178、1.1874、0.1133和0.1752;方差分析显示各位点的Nei’s基因多样度和香农信息指数具有不同程度的显著性差异;基于Lei&Li相似系数利用UPGMA法进行聚类分析,在相似系数0.786处将25个品种划分为4大类。根据AFLP结果探讨了山东石榴品种的亲缘关系与新品种选育的前景。更多还原

【Abstract】 The genetic diversity of 25 pomegranate cultivars cultivated in Shandong Province was ana- lyzed by the fluorescent-AFLP marker techniques with 8 pairs of EcoR I/Mse | primer combinations.The re- suits showed that the number of polymorphic loci amplified by 8 pairs of fluorescent-AFLP primers in 25 culti- vars were 70 to 126,the average was 90.25,the mean percentage of polymorphic loci was 41.78%.All aver- age observed number of alleles,average effective number of alleles,average Nei’s gene diversity index,and average Shannon’s information index were 1.4178,1.1874,0.1133 and 0.1752 respectively;Variance analysis’s results indicated that there was a significant difference in Nei’s gene diversity index and Shannon’s information index of each locus.Cluster analysis was performed by using of UPGMA method based on Lei & Li similarity coefficients,and 25 pomegranate cuhivars in Shandong were divided into 4 groups at O.786 level of similarity coefficient.In addition the relationship in Shandong pomegranate cultivars and the foreground of breeding were discussed based on the results of AFLP analysis.更多还原