【摘要】 以盆栽枳椇和皂荚为试材,研究了不同浓度NaCl处理(CK、0.15%、0.30%、0.45%、0.60%)对枳椇和皂荚植株生长、叶绿素荧光特性和活性氧代谢的影响.结果表明:随着NaCl浓度的提高,植株生长量、叶片叶绿素含量、Fv/Fm、ΦPSⅡ、qP均逐渐下降,而qN逐渐升高.0.15%NaCl胁迫10d后,枳椇叶片叶绿素含量、Fv/Fm、ΦPSⅡ、qP较对照处理分别降低了19.77%、2.94%、29.03%、8.16%,差异显著(P<0.05),而皂荚与对照处理差异不显著;0.30%NaCl胁迫下,皂荚Fv/Fm和ΦPSⅡ较对照处理分别降低了1.91%、14.66%,差异显著(P<0.05);0.45%NaCl胁迫下,皂荚叶片叶绿素含量和qP与对照处理差异显著(P<0.05),分别降低了29.28%和11.36%.随着NaCl浓度的增加,皂荚SOD活性持续升高,枳椇呈先升高后降低变化,在0.15%NaCl胁迫下达最大值;两种苗木细胞POD、CAT活性均呈先升后降趋势,且皂荚的升幅大于枳椇.二者的MDA含量均呈上升趋势,且枳椇的升高幅度大于皂荚,说明枳椇产生了较严重的膜脂过氧化.由此推断,皂荚的耐盐性较强与其具有较强的抗氧化保护酶系统有关.更多还原

【Abstract】 With potted Hovenia dulcis and Gleditsia sinensis seedlings as test materials,their plant growth,chlorophyll fluorescence characteristics,and active oxygen metabolism under stress of different concentration(0,0.15%,0.30%,0.45%,and 0.60%) NaCl were studied.The results showed that with increasing concentration of NaCl,the plant growth,leaf chlorophyll content,photochemical efficiency of PSⅡ(Fv/Fm),quantum yield of PSⅡ(ΦPSⅡ),and photochemical quenching(qP) decreased gradually,while the non-photochemical quenching of fluorescence(qN) was in adverse.After 10 days of 0.15% NaCl stress,the leaf chlorophyll content,Fv/Fm,ΦPSⅡ,and qP of H.dulcis seedlings decreased by 19.77%,2.94%,29.03%,and 8.16%,respectively,with significant differences(P<0.05) to the control,while no significant differences were observed for G.sinensis seedlings.Compared with the control,the Fv/Fm and ΦPSⅡ of G.sinensis seedlings in treatment 0.30% NaCl decreased significantly by 1.91% and 14.66%,and the chlorophyll content and qP of the seedling in treatment 0.45% NaCl decreased significantly by 29.28% and 11.36%,respectively(P<0.05).With increasing concentration of NaCl,the SOD activity of G.sinensis seedlings showed a consistent increasing trend,and that of H.dulcis seedlings increased first and decreased then.The POD and CAT activities of G.sinensis and H.dulci seedlings tended to increase first and decrease then,with the increment being higher for G.sinensis than for H.dulci,while the MDA content of the seedlings had an increasing trend,with the increment being higher for H.dulcis than for G.sinensis,suggesting that the cell membrane lipid peroxidation of H.dulcis was more serious than that of G.sinensis.It was concluded that G.sinensis had greater salt tolerance than H.dulcis,which was related to its higher anti-oxidation enzyme activities.更多还原